US 2004/0171015 A1 · Cyp450-specific dna probes and primers, and biological applications thereof

Abstract

The present application relates to a set of new cDNA probes which enables the specific and simultaneous detection of the main fourteen CYP450 isoforms, and to new primers specific to the probes. It also provides transfection vectors, genetically engineered cells and with amplification products derived therefrom. The present application notably encompasses any solid surface such as DNA filters or DNA chips which comprises a cDNA probe or an amplification product of the invention. The products provided thereby enables to evaluate the toxicity or pathogenicity of a product, and to predict drug in vivo interactions or efficiency.

Claims19

Dependent claims

Isolated and purified polynucleotide selected from the group consisting of SEQ ID NO:

1, SEQ ID NO:
2, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
5, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
11, SEQ ID NO:
12, SEQ ID NO:
13, SEQ ID NO:
14, and the complementary sequences thereof.

11 more elements

Prime pair selected from the group consisting of

SEQ ID NO:
15 and SEQ ID NO:
16,
SEQ ID NO:
17 and SEQ ID NO:
18,
SEQ ID NO:
19 and SEQ ID NO:
20,
SEQ ID NO:
21 and SEQ ID NO:
22,
SEQ ID NO:
23 and SEQ ID NO:
24,
SEQ ID NO:
25 and SEQ ID NO:
26,
SEQ ID NO:
27 and SEQ ID NO:
28,
SEQ ID NO:
29 and SEQ ID NO:
30,
SEQ ID NO:
31 and SEQ ID NO:
32,
SEQ ID NO:
33 and SEQ ID NO:
34,
SEQ ID NO:
35 and SEQ ID NO:
36,
SEQ ID NO:
37 and SEQ ID NO:
38,
SEQ ID NO:
39 and SEQ ID NO:
40,
SEQ ID NO:
41 and SEQ ID NO:
42 and
their respective complementary oligonucleotide couples.

40 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/480,276Filed 11 Dec 2003

Published

2 Sep 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
C07K 14/80
Porphyrin- or corrin-ring-containing peptides: cytochromes
C12Q 2600/106
Oligonucleotides characterized by their use: pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
C12Q 2600/156
Oligonucleotides characterized by their use: polymorphic or mutational markers

Cyp450-specific dna probes and primers, and biological applications thereof

Life of this patent

Cyp450-specific dna probes and primers, and biological applications thereof

Life of this patent

Abstract

Claims19