Immobilized asymmetric double lipid layers
OwnerJohannes Schmitt, Joachim Nollerinventors
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Abstract
The invention relates to a method suitable for the production of immobilized double lipid layers, comprising an inner and an outer lipid layer. The inner and outer lipid layer of said double lipid layers have a defined orientation on the surface of a solid body and are immobilized on the surface of said solid body in a substantially stable and dynamic manner. In order to carry out the inventive method, the surface of a solid body is initially modified in such a way that a surface is formed which preferably interacts with only one of the two lipid layers. In a second step, a double lipid layer is deposited on said modified surface. Preferably, other components, especially protein, are inserted into the double lipid layer.
Claims22
A process for preparing an immobilized lipid bilayer having an inner lipid layer and an outer lipid layer, with the lipid bilayer exhibiting a defined orientation in regard to the lipid layers on a surface of a solid and being immobilized in an essentially stable and dynamic manner on the surface of the solid, comprising the following process steps:
- a) modifying the surface of the solid in order to form a surface which preferably interacts with only one of the two lipid layers, and
- b) depositing the lipid bilayer on the modified surface.
The process as claimed in one of the preceding claims, characterized in that the modification of the surface of the solid comprises the following constituent steps:
- aa) functionalizing the surface and/or
- ab) adsorbing and/or chemisorbing interacting molecules.
The process as claimed in one of the preceding claims, characterized in that the surface which has been modified in accordance with process step
- a) interacts with the inner lipid layer.
The use of a modified surface of a solid for preparing an immobilized lipid bilayer having an inner lipid layer and an outer lipid layer, with the lipid bilayer exhibiting a defined orientation in regard to the lipid layers on a surface of a solid and being immobilized in an essentially stable and dynamic manner on the surface of the solid.
A kit for preparing immobilized lipid bilayers having inner and outer lipid layers, with the lipid bilayers exhibiting a defined orientation in regard to the lipid layers on the surface of solids and being immobilized in an essentially stable and dynamic manner on the surface of the solids, at least comprising a solid having a modified surface which preferably interacts with only one of the two lipid layers.
A kit for preparing immobilized proteins in immobilized lipid bilayers having inner and outer lipid layers, with the lipid bilayers exhibiting a defined orientation in regard to the lipid layers on the surface of solids and being stabilized in an essentially stable and dynamic manner on the surface of the solids, at least comprising a solid having a modified surface, which preferably interacts with only one of the two lipid layers, and also, preferably, lipid vesicles which exhibit proteins.
A process for investigating proteins, in particular membrane proteins, characterized in that these proteins are employed as immobilized proteins in immobilized lipid bilayers having inner and outer lipid layers, with the lipid bilayers exhibiting a defined orientation in regard to the lipid layers on the surface of solids and being immobilized in an essentially stable and dynamic manner on the surface of the solids.
Dependencies are inferred from the claim text.