G-protein coupled receptor arrays

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has less than 90% intrafamily identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has less than 85% intrafamily identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has less than 80% intrafamily identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has less than 75% intrafamily identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has less than 50% intrafamily identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has at least 50% interspecies identity.

The array according to any of the preceding claims, wherein the non-conserved regions of the GPCR polynucleotide family member has an average length of from about 3 to about 9,000 nucleotides, from about 3 to about 6,000, from about 3 to about 3,000, from about 200 to 750, from about 200 to 700, from about 200 to 500, from about 250 to 400 or from about 290 to 350.

The array according to any of the preceding claims, wherein said different regions of a GPCR polynucleotide family member are polynucleotide regions from the same polynucleotide strand and the regions are at least non-overlapping polynucleotide regions of the strand.

The array according to any of the preceding claims, wherein a GPCR polynucleotide family is polynucleotides encoding polypeptides capable of transducing a signal across biological membranes.

The array according to any of the preceding claims, wherein the GPCR polynucleotide family is selected from the group consisting of GPCR's belonging to any of the overall groups of Rhodopsin-like receptors, secretin-like, metabotropic glutamate/pheromone, Frizzled/smoothened and a large groups of unclassified GPCR's, which are all characterized by they seven transmembrane spanning regions and their ability to transduce a signal across a cellular membrane.

The array according to any of the preceding claims, wherein the polynucleotide composition comprises one or more of the same non-conserved region of a GPCR polynucleotide family member.

The array according to any of the preceding claims, wherein the non-conserved region of a GPCR polynucleotide family member is of DNA, RNA, cDNA, natural, synthetic, semi-synthetic origin or is a chemical analogous such as LNA and PNA.

The array according to any of the preceding claims, wherein the non-conserved region of a GPCR polynucleotide family member is obtained from one or more biological materials such as e.g. an organism, an organ, a tissue, a cell or a biological material produced by a cell culture.

The array according to any of the preceding claims, wherein the solid support is made of a flexible or rigid material.

The array according to any of the preceding claims, wherein said array comprises from about 2 to about 100,000 such as, e.g. from about 2 to about 50,000, from about 10 to about 25,000, from about 100 to about 10,000, from about 100 to about 5,000, from about 100 to about 1,000, from about 400 to about 600 or about 500 GPCR polynucleotide spots, or at least 2 such as, e.g. at least 10, at least 25, at least 50, at least 100, at least 300, at least 400, at least 500 or at least 600 spots.

A method of preparing an array according to any of the preceding claims, said method comprising a) generating said non-conserved regions of GPCR polynucleotide family members, b) preparing a multiplicity of compositions each comprising at least a non-conserved region, and c) stably associating said compositions in individual spots on a surface of a solid support.