Detection of negatively charged polymers using water-soluble, cationic, polythiophene derivatives
AssigneesInfectio Diagnostic (I.D.I.), Inc., Universite Laval
InventorsMario Leclerc, Hoang Ahn Ho, Maurice Boissinot
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Abstract
Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly (3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hibridized) oligonucleotides.
Claims41
A thiophene monomer having the following general formula:
- 9
- wherein:
- a) “m” is an integer ranging from 2 to 3;
- b) R* is a quaternary ammonium;
- c) Y is an oxygen atom or a methylene;
- and
- d) R1 is a methyl group or a hydrogen atom.
Use of a positively charged polymer comprising a repeating thiophene moiety for detecting the presence of a negatively charged polymer.
Dependencies are inferred from the claim text.