Diagnostic polymorphisms of tgf-beta1 promoter
OwnerDavid W. Moskowitzinventor
Life of this patent
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Abstract
Disclosed are single nucleotide polymorphisms (SNPs) associated with hypertension and end stage renal disease due to hypertension. Also disclosed are methods for using SNPs to determine susceptibility to end stage renal disease and hypertension; nucleotide sequences containing SNPs; kits for determining the presence of SNPs; and methods of treatment or prophylaxis based on the presence of SNPs.
Claims50
A method for diagnosing a genetic susceptibility for a disease, condition, or disorder in a subject comprising:
- obtaining a biological sample containing nucleic acid from said subject;
- and
- analyzing said nucleic acid to detect the presence or absence of a single nucleotide polymorphism in the TGF-β1 gene, wherein said single nucleotide polymorphism is associated with a genetic susceptibility for hypertension.
An isolated polynucleotide comprising at least 10 contiguous nucleotides of SEQ ID NO:
- 1, or the complements thereof, and containing at least one single nucleotide polymorphism at position 474, 510, 546, or 563 of SEQ ID NO:
- 1 wherein said at least one single nucleotide polymorphism is associated with hypertension.
A kit comprising at least one isolated polynucleotide of at least 10 contiguous nucleotides of SEQ ID NO:
- 1 or the complement thereof, and containing at least one single nucleotide polymorphism associated with hypertension;
- and instructions for using said polynucleotide for detecting the presence or absence of said at least one single nucleotide polymorphism in said nucleic acid.
A kit comprising at least one polynucleotide of at least 10 contiguous nucleotides of SEQ ID NO:
- 1 or the complement thereof, wherein the 3′ end of said polynucleotide is immediately 5′ to a single nucleotide polymorphism site associated with hypertension;
- and instructions for using said polynucleotide for detecting the presence or absence of said single nucleotide polymorphism in a biological sample containing nucleic acid.
A method for treatment or prophylaxis in a subject comprising:
- obtaining a sample of biological material containing nucleic acid from a subject;
- analyzing said nucleic acid to detect the presence or absence of at least one single nucleotide polymorphism in SEQ ID NO:
- 1 or the complement thereof associated with hypertension;
- and
- treating said subject for said disease, condition or disorder.
A method for diagnosing a genetic susceptibility for a disease, condition, or disorder in a subject comprising:
- obtaining a biological sample containing nucleic acid from said subject;
- and
- analyzing said nucleic acid to detect the presence or absence of a single nucleotide polymorphism in the TGF-βI gene, wherein said single nucleotide polymorphism is associated with a genetic susceptability for end stage renal disease due to hypertension.
An isolated polynucleotide comprising at least 10 contiguous nucleotides of SEQ ID NO:
- 1, or the complements thereof, and containing at least one single nucleotide polymorphism at position 474, 510, or 546 of SEQ ID NO:
- 1 wherein said at least one single nucleotide polymorphism is associated with end stage renal disease due to hypertension.
A kit comprising at least one isolated polynucleotide of at least 10 contiguous nucleotides of SEQ ID NO:
- 1 or the complement thereof, and containing at least one single nucleotide polymorphism associated with end stage renal disease due to hypertension;
- and instructions for using said polynucleotide for detecting the presence or absence of said at least one single nucleotide polymorphism in said nucleic acid.
A kit comprising at least one polynucleotide of at least 10 contiguous nucleotides of SEQ ID NO:
- 1 or the complement thereof, wherein the 3′ end of said polynucleotide is immediately 5′ to a single nucleotide polymorphism site associated with end stage renal disease due to hypertension;
- and instructions for using said polynucleotide for detecting the presence or absence of said single nucleotide polymorphism in a biological sample containing nucleic acid.
A method for treatment or prophylaxis in a subject comprising:
- obtaining a sample of biological material containing nucleic acid from a subject;
- analyzing said nucleic acid to detect the presence or absence of at least one single nucleotide polymorphism in SEQ ID NO:
- 1 or the complement thereof associated with end stage renal disease due to hypertension;
- and
- treating said subject for said disease, condition or disorder.
Dependencies are inferred from the claim text.