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US 2004/0170991 A1Published application
Method for removing amplification inhibitors from test samples
AssigneeAbbott Laboratories
InventorsFolim G. Halaka, Dwight Erickson, Qizhi He, Gregor W. Leckie, Bor-Chian Lin
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Abstract
Provided herein is a method for removing nucleic acid amplification reaction inhibitors from test samples.
Claims10
A method for alleviating inhibition in nucleic acid amplification assays comprising the steps of:
- a) providing a test sample comprising a liquid and a target sequence;
- b) acidifying said test sample to place said target sequence in a second liquid;
- and
- c) replacing said second liquid with a buffer suitable for nucleic acid amplification.
1 more element
In a method for preparing a test sample for nucleic acid amplification comprising the steps of:
- a) treating said sample with a basic solution to thereby form a basic test sample;
- and
- b) placing any target sequences contained in said test sample into a buffer suitable for nucleic acid amplification;
- wherein the improvement comprises acidifying said basic test sample after step a) and before step b).
1 more element
Dependencies are inferred from the claim text.