Intracellular nucleic acid inhibitors of small guanine nucleotide exchange factors
OwnerKolanus Waldemar, Michael Famulok +2inventors
InventorsKolanus Waldemar, Michael Famulok, Michael Blind, Gunter Mayer
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Abstract
The invention relates to a nucleic acid, especially an isolated nucleic acid, that is capable of binding to a guanine nucleotide exchange factor for ADP ribosylation factors, and to the derivatives thereof.
Claims30
A nucleic acid, particularly an isolated nucleic acid which is capable of binding to a guanine nucleotide exchange factor for ADP ribosylation factors, and its derivatives.
A nucleic acid, particularly an isolated nucleic acid which is capable of binding to a guanine nucleotide exchange factor for ADP ribosylation factors, characterised in that the nucleic acid comprises a sequence selected from the group comprising SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 18, SEQ ID No. 19, SEQ ID No. 20, SEQ ID No. 21, SEQ ID No. 22, SEQ ID No. 23, SEQ ID No. 24, SEQ ID No. 25, SEQ ID No. 26, SEQ ID No. 27, SEQ ID No. 28 SEQ ID No. 29, SEQ ID No. 30, SEQ ID No. 31, SEQ ID No. 32, SEQ ID No. 33, SEQ ID No. 34, SEQ ID No. 35, SEQ ID No. 36, SEQ ID No. 37, SEQ ID No. 38 and SEQ ID No. 39 as well as its respective derivatives.
An inhibitor for a guanine nucleotide exchange factor, wherein the guanine nucleotide exchange factor belongs to the group of small guanine nucleotide exchange factors for ARF proteins, particularly to the group of guanine nucleotide exchange factors whose molecular weight is around 50 kDa or less.
Use of a guanine nucleotide exchange factor as a target molecule as part of an in vitro selection process.
A method for identifying and isolating nucleic acids capable of binding to a target molecule, characterised by the following steps:
- incubating the target molecule or a part thereof with a plurality of nucleic acids, preferably a nucleic acid library, wherein the nucleic acids have different sequences,
- selecting and isolating those nucleic acids capable of binding to the target molecule or a part thereof,
- optionally amplifying the isolated nucleic acids and repeating the first two steps;
- and
- optionally determining the sequence and/or binding specificity of the isolated nucleic acids,
- characterised in that the target molecule is a guanine nucleotide exchange factor, preferably one such for ARF.
Use of a nucleic acid according to any one of the preceding claims, preferably in a complex with a guanine nucleotide exchange factor, for the rational design of compounds, preferably of inhibitors and more preferably of low-molecular inhibitors.
Dependencies are inferred from the claim text.