Mixed cell diagnostic systems
AssigneesUniversity Hospitals of Cleveland, Diagnostic Hybrids, Inc.
InventorsDavid R. Scholl, Patricia Gail Ray Goodrum, Yung T. Huang
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Abstract
The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of a organisms to antimicrobial agents.
Claims34
A composition comprising a mixed cell culture comprising MDCK cells and one or more of A549 cells and H292 cells.
A method for detecting influenza virus, comprising:
- 1) providing:
- a) mixed cell culture comprising MDCK cells and one or more of A549 cells and H292 cells;
- and
- b) a sample;
- 2) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- 3) detecting the presence of influenza virus in said inoculated culture.
A method for producing influenza virus, comprising:
- 1) providing:
- a) mixed cell culture comprising MDCK cells and one or more of A549 cells and H292 cells;
- and
- b) a sample;
- and
- 2) inoculating said mixed cell culture with said sample to produce an inoculated culture, wherein said inoculated culture produces influenza virus.
A method for detecting metapneumovirus, comprising:
- 1) providing
- a) a mixed cell culture comprising MDCK cells and A549 cells;
- and
- b) a sample;
- 2) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- 3) detecting the presence of metapneumovirus in said inoculated culture.
32 The method of claim 31, wherein said method further comprises detecting the presence of one or more of respiratory syncytial virus (RSV), adenovirus, parainfluenza 1 virus, parainfluenza 2 virus, and parainfluenza 3 virus.
A method for producing metapneumovirus, comprising:
- 1) providing
- a) a mixed cell culture comprising MDCK cells and A549 cells;
- and
- b) a sample;
- 2) inoculating said cultured cells with said sample to produce an inoculated culture, wherein said inoculated culture produces metapneumovirus.
Dependencies are inferred from the claim text.