Composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces
AssigneeUniverstiy of Utah Research Foundation
InventorsKarin D. Caldwell, Patrick A. Tresco, Jennifer Neff
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Abstract
The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
Claims41
A method for the attachment of cells, viruses, proteins, or polypeptides for growth or biological analysis comprising the steps of:
- conjugating a polypeptide to a PEO- and PPO- containing triblock and diblock copolymer comprising an activated end group to form a polypeptide-conjugated copolymer;
- contacting a hydrophobic surface with said polypeptide-conjugated copolymer for a time sufficient for said polypeptide-conjugated copolymer to be adsorbed by the hydrophobic surface, thereby forming a polypeptide-conjugated copolymer-coated surface;
- and
- contacting said polypeptide-conjugated copolymer-coated surface with at least one cell, virus, protein, or polypeptide such that said cell, virus, protein, or polypeptide adheres to said polypeptide-conjugated copolymer-coated surface.
A method for the attachment of cells for growth or biological analysis comprising the steps of:
- a) conjugating a natural or recombinant biomolecule to a PEO- and PPO containing triblock or diblock copolymer comprising an activated end group to form a biomolecule-conjugated copolymer;
- b) contacting a hydrophobic surface with said biomolecule-conjugated copolymer for a time sufficient for said biomolecule-conjugated copolymer to be adsorbed onto the hydrophobic surface thereby forming a biomolecule-conjugated copolymer-coated surface;
- and
- c) contacting said biomolecule-conjugated copolymer-coated surface with at least one cell such that said cell adheres to the biomolecule-conjugated copolymer-coated surface.
A method for the attachment of cells or viruses to a surface for growth or biological analysis comprising the steps of:
- a) obtaining a PEO- and PPO- containing triblock or diblock copolymer, wherein said copolymer comprises an end group;
- b) activating the end group of said copolymer;
- c) conjugating a thiol containing biomolecule to said copolymer to from a biomolecule-conjugated copolymer;
- d) contacting a hydrophobic surface with said biomolecule-conjugated copolymer to form a biomolecule-conjugated copolymer-coated surface;
- and
- e) contacting the biomolecule-conjugated copolymer-coated surface with a cell or virus such that said cell or virus adheres to the biomolecule-conjugated copolymer-coated surface.
Dependencies are inferred from the claim text.