Methods for identifying antiviral oligonucleotides
AssigneeReplicor, Inc.
InventorsAndrew Vaillant, Jean-Marc Juteau
Life of this patent
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Abstract
Random sequence oligonucleotides that have antiviral activity are described, along with their use as antiviral agents. In many cases, the oligonucleotides are greater than 40 nucleotides in length. Also described are methods for the prophylaxis or treatment of a viral infection in a human or animal, and a method for the prophylaxis treatment of cancer caused by oncoviruses in a human or animal. The methods typically involve administering to a human or animal in need of such treatment, a pharmacologically acceptable, therapeutically effective amount of at least oligonucleotide that does not act by a sequence complementary mode of action.
Claims32
A method of screening to identify a compound that alters binding of an oligonucleotide to at least one viral component, said method comprising
- in separate reactions, contacting said oligonucleotide with said viral component in the presence and absence of a compound to be screened;
- and
- determining whether a difference occurs in binding of said oligonucleotide to said viral component in the presence of said compound compared to in the absence of said compound, the presence of said difference being indicative of said compound altering the binding of said oligonucleotide to said viral component.
A method for purifying oligonucleotides binding to at least one viral component from a pool of oligonucleotides comprising:
- contacting said pool with at least one viral component;
- displacing bound oligonucleotides of said pool from said component;
- and
- collecting displaced oligonucleotides.
A method for enriching oligonucleotides from a pool of oligonucleotides binding to at least one viral component, comprising:
- contacting said pool with at least one viral component;
- and
- amplifying oligonucleotides bound to said viral components to provide an enriched oligonucleotide pool.
Dependencies are inferred from the claim text.