Method for identifying targeting domains and methods and compositions comprising the same
AssigneeGeorgetown University
InventorsAnton Wellstein, Kevin J. McDonnell, Justinian R. Ngaiza
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Abstract
The present invention is directed to a method of identifying tissue targeting domains. In particular, the invention relates to methods for identifying a polynucleotide encoding a targeting domain which directs tumor cell localization to secondary sites, to methods of utilizing the polynucleotide and corresponding polypeptide or fragments thereof and compositions comprising the same.
Claims37
A method of identifying a ploynucleotide encoding a targeting domain associated with metastasis, the method comprising:
- a) injecting phage displaying a collection of polypeptides expressed from nucleic acid sequences that are derived from a eukaryotic cell, into a subject;
- b) selecting phage that localize in a target organ or tissue;
- c) collecting phage from the selected organ or tissue
- d) repeating steps (a) and (c) for one or more cycles;
- and
- e) identifying one or more polynucleotides encoding targeting domains from selected phage that are associated with metastasis.
An isolated polynucleotide that hybridizes under stringent conditions to a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1-9 and combinations thereof.
An isolated polynucleotide that encodes a polypeptide comprising one or more of the polypeptide sequences selected from the group consisting of SEQ ID NOS:
- 10-36 and combinations thereof.
A polypeptide comprising one or more of the polypeptide sequences selected from the group consisting of SEQ ID NOS:
- 10-36 and combinations thereof.
A method of treating metastasis comprising administering to a subject in need of such treatment a targeting domain operably linked to a therapeutic agent in an amount effective to treat the metastasis.
Dependencies are inferred from the claim text.