Human and mouse targeting peptides identified by phage display

A method of preparing a phage display library comprising:

• a) immunizing a host animal with a target organ, tissue or cell type;
• b) obtaining mRNAs encoding antibodies from the host animal;
• c) preparing cDNAs from the mRNAs encoding antibodies;
• and
• d) preparing a phage display library from the cDNAs.

A method of delivering an agent to a fetus comprising:

• a) obtaining a peptide comprising at least three contiguous amino acids of a sequence selected from SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44 or SEQ ID NO:45;
• b) attaching the peptide to an agent;
• and
• b) administering the peptide to a pregnant subject.

A method of targeting delivery to adipose tissue comprising:

• a) obtaining a targeting peptide comprising an amino acid sequence of at least three contiguous amino acids selected from SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54 or SEQ ID NO:55;
• b) attaching the peptide to an agent to form a complex;
• and
• c) administering the complex to a subject.

A method comprising;

• a) injecting a subject with a phage display library;
• b) recovering at least one sample of at least one organ, tissue or cell type;
• c) separating the sample into isolated cells or clumps of cells;
• d) centrifuging the cells through an organic phase to form a pellet;
• and
• e) recovering phage from the pellet.

A method of targeting delivery to prostate cancer comprising:

• a) obtaining a targeting peptide comprising at least three contiguous amino acids selected from any of SEQ ID NO:20 through SEQ ID NO:38;
• b) attaching the peptide to a therapeutic agent to form a complex;
• and
• c) administering the complex to a subject with prostate cancer.

A method of diagnosing prostate cancer comprising:

• a) obtaining a targeting peptide comprising at least three contiguous amino acids selected from any of SEQ ID NO:20 through SEQ ID NO:38;
• b) administering the peptide to a subject suspected of having prostate cancer;
• and
• c) detecting the peptide bound to prostate cancer cells.

A method of identifying targeting peptides to angiogenic tissue comprising:

• a) inducing hypoxia in a neonatal subject;
• b) administering a phage display library to the subject;
• and
• c) recovering phage from the retina of the subject.

A method of inducing apoptosis in a cell comprising:

• a) obtaining a targeting peptide comprising at least three contiguous amino acids selected from any of SEQ ID NO:93 through SEQ ID NO:121;
• b) attaching the peptide to a permeabilizing agent to form a complex;
• and
• c) administering the complex to the cell.

A method of targeting delivery to an angiogenic tissue comprising:

• a) obtaining a peptide comprising at least three contiguous amino acids selected from SEQ ID NO:123, SEQ ID NO:125, SEQ ID NO:
• 126, SEQ ID NO:127, SEQ ID NO:128, SEQ ID NO:129, SEQ ID NO:130 or SEQ ID NO:131;
• b) attaching the peptide to a therapeutic agent to form a complex;
• and
• b) administering the complex to a subject.

A method of detecting receptors for endostatin or angiostatin comprising

• a) obtaining a sample from a tissue or organ;
• b) incubating the sample with endostatin or angiostatin;
• and
• c) detecting the presence of endostatin or angiostatin bound to the sample.