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US 2004/0170646 A1Published application
Production of ALVAC on avian embryonic stem cells
AssigneeAventis Pasteur Limited
InventorsVeronique Barban, Luc Aujame
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Abstract
The present invention relates to methods for producing ALVAC virus on avian embryonic stem cells and compositions comprising ALVAC virus made using such methods.
Claims28
A method for propagating an ALVAC virus, comprising:
- (a) infecting one or mores avian embryonic stem cells with an ALVAC virus;
- (b) cultivating the infected avian embryonic stem cells to produce the virus;
- and (c) isolating the virus.
A method for propagating a virus, comprising:
- (a) infecting one or more cells derived from an avian embryonic stem cell with an ALVAC virus;
- (b) cultivating the infected cells to produce the virus;
- and, (c) isolating the virus.
A method for preparing a immunogenic composition comprising:
- (a) infecting avian embryonic stem cells with an ALVAC virus comprising within the ALVAC genome at least one exogenous nucleotide sequence encoding a human tumor antigen, an antigen derived from a human pathogen, or a fragment thereof;
- (b) cultivating the infected cells to produce the virus;
- (c) harvesting the virus from the cultivated cells;
- and, (d) subjecting the harvested virus to at least one of the following treatments:
- (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, or (iv) lyophilization.
2 more elements
The method of claim 17 wherein the ALVAC virus is ALVAC(2).
A composition comprising an ALVAC virus produced by the method of claim 17 or 18.
A method for preparing a immunogenic composition comprising:
- (a) infecting one or more cells derived from an avian embryonic stem cell with an ALVAC virus comprising within the ALVAC genome at least one exogenous nucleotide sequence encoding an antigen derived from a human tumor antigen, an antigen derived from a human pathogen, or a fragment thereof;
- (b) cultivating the infected cells to produce the virus;
- (c) harvesting the virus from the cultivated cells;
- and, (d) subjecting the harvested virus to at least one of the following treatments:
- (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, or (iv) lyophilization.
2 more elements
A method for providing a vaccine to a host, comprising:
- (a) infecting avian embryonic stem cells with an ALVAC virus having within the ALVAC genome at least one exogenous nucleotide sequence encoding a human tumor antigen, an antigen derived from a human pathogen, or a fragment thereof;
- (b) cultivating the infected cells to produce the virus;
- (c) harvesting the virus from the cultivated cells;
- (d) subjecting the harvested virus to at least one of the following treatments:
- (i) inactivating the virus, (ii) adding a pharmaceutically acceptable carrier or diluent, (iii) adding an adjuvant, (iv) adding a stabilizer, or (v) lyophilizing to produce a vaccinal composition;
- and, (e) administering the vaccinal composition to the host whereby a protective immune response occurs in the host.
3 more elements
Dependencies are inferred from the claim text.