Production of peptides in plants as viral coat protein fusions
OwnerKenneth E. Palmer, Long V. Nguyen +6inventors
InventorsKenneth E. Palmer, Long V. Nguyen, Rachel L. Toth, Michael Jones, Sean Chapman, Lisa Smolenska, Alison A. McCormick, Gregory P. Pogue
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Abstract
Vaccines and diagnostic composition are made and used for preventing, treating and detecting antigens from a papilloma virus, ebola virus, HIV virus, Rift Valley Fever virus or a parvovirus. The epitopes of these viruses are produced as genetically engineered fusion peptides in plants by infection with a recombinant tobamovirus vectors to express fusion proteins containing the epitope peptides.
Claims18
An immunological reagent comprising a plant viral protein covalently bound to an epitope peptide having the same linear sequence as an immunologically recognized epitope of a human papilloma virus, human immunodeficiency virus, ebola virus, rift valley fever virus or parvovirus.
A virus-like particle comprising a plurality of assembled protein subunits wherein each protein subunit is a plant viral coat protein covalently bound to an epitope peptide having the same linear sequence as an immunologically recognized epitope of a human papilloma virus, human immunodeficiency virus, ebola virus, rift valley fever virus or parvovirus.
A plant virus comprising at least one plant viral coat protein covalently bound to an epitope peptide having the same linear sequence as an immunologically recognized epitope of a human papilloma virus, human immunodeficiency virus, ebola virus, rift valley fever virus or parvovirus.
The method of 14 wherein the antibody is neutralizing.
A method for inducing an immune response in an animal against a peptide epitope comprising
- coupling the peptide epitope to a first carrier antigen to make a first vaccine composition,
- coupling the peptide epitope to a second carrier antigen, which is different from the first carrier antigen, to make a second vaccine composition,
- immunizing the animal with the first vaccine composition,
- at a later time, immunizing the animal with the second vaccine composition,
- wherein the immune response to the peptide epitope is boosted greater than the boosting of either carrier antigen.
Dependencies are inferred from the claim text.