IL-6 receptor IL-6 direct fusion protein
AssigneeTosoh Corporation
InventorsTeiji Ekida, Harutaka Yagame, Hiroshi Iida, Kiyoshi Yasukawa, Shigeo Tsuchiya, Teruhiko Ide
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Abstract
The present invention intends to provide an IL-6R•IL-6 fusion protein and the like in which IL-6R and IL-6 are directly linked without a linker. The IL-6 receptor•IL-6 fusion protein of the present invention has a structure in which one amino acid residue constituting IL-6 receptor and one amino acid residue constituting IL-6 are directly bonded.
Claims16
An IL-6 receptor•IL-6 fusion protein, in which one amino acid residue constituting IL-6 receptor and one amino acid residue constituting IL-6 are directly linked.
An IL-6 receptor•IL-6 fusion protein, in which C-terminal of any one of 39 amino acid residues of from N-terminal 323th alanine residue to N-terminal 361th serine residue of IL-6 receptor is linked to an N-terminal amino acid residue of IL-6.
A gene for coding for IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6R and one amino acid residue of IL-6 are linked directly.
A yeast of Pichia pastoris species which is transformed by an expression vector containing a gene for coding for IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are linked directly.
A process for producing an IL-6 receptor•IL-6 fusion protein, comprising a step of cultivating in a culture medium a yeast of a Pichia pastoris species having been transformed by an expression vector containing a gene for coding for an IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are linked directly;
- and a step of collecting the IL-6 receptor•IL-6 fusion protein as a secretory protein from the culture medium.
A process for producing an IL-6 receptor•IL-6 fusion protein, comprising a step of cultivating a yeast of a Pichia pastoris species having been transformed by an expression vector containing a gene for coding for an IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are linked directly, in a culture medium of natural origin containing a carbon source and no methanol, and adding methanol during progress of the cultivation.
A process for producing an IL-6 receptor•IL-6 fusion protein, comprising a step of cultivating a yeast of a Pichia pastoris species having been transformed by an expression vector containing a gene for coding for an IL-6 receptor•IL-6 fusion protein in which C-terminal of any one of 39 amino acid residues of from N-terminal 323th alanine residue to N-terminal 361th serine residue of IL-6 receptor is linked to an N-terminal amino acid residue of IL-6.
A process for producing an IL-6 receptor•IL-6 fusion protein, comprising collecting the IL-6 receptor•IL-6 fusion protein by treating a solution containing IL-6 receptor•IL-6 fusion protein, in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are linked directly, by three kinds of chromatography including ion-exchange chromatography, hydrophobic chromatography, and gel-filtration chromatography to collect the IL-6 receptor•IL-6 fusion protein.
A process for producing an IL-6 receptor•IL-6 fusion protein, comprising collecting the IL-6 receptor•IL-6 fusion protein by treating a solution containing IL-6 receptor•IL-6 fusion protein, in which C-terminal of any one of 39 amino acid residues of from N-terminal 323th alanine residue to N-terminal 361th serine residue of IL-6 receptor is linked to an N-terminal amino acid residue of IL-6, by three kinds of chromatography including ion-exchange chromatography, hydrophobic chromatography, and gel-filtration chromatography to collect the IL-6 receptor•IL-6 fusion protein.
An ex vivo amplifier for a hematopoietic stem cells, comprising an IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are directly linked.
An ex vivo amplifier for a hematopoietic stem cells, comprising an IL-6 receptor•IL-6 fusion protein in which C-terminal of any one of 39 amino acid residues of from N-terminal 323th alanine residue to N-terminal 361th serine residue of IL-6 receptor is linked to an N-terminal amino acid residue of IL-6.
A blood platelet proliferating agent containing as a main component an IL-6 receptor•IL-6 fusion protein in which one amino acid residue of IL-6 receptor and one amino acid residue of IL-6 are directly linked.
A blood platelet proliferating agent containing as a main component an IL-6 receptor•IL-6 fusion protein in which C-terminal of any one of 39 amino acid residues of from N-terminal 323th alanine residue to N-terminal 361th serine residue of IL-6 receptor is linked to an N-terminal amino acid residue of IL-6.
Dependencies are inferred from the claim text.