Method for tracking particles and life forms in three dimensions and in time
AssigneeDalhousie University
InventorsH. Juergen Kreuzer, Manfred H. Jericho
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Abstract
A method for tracking particles and life forms in three dimensions and in time. The present invention applies a numerical reconstruction approach to digital in-line holographic microscopy images in order to generate a time sequence hologram representing the trajectory of objects such as particles and life forms. By subtracting consecutive (in time) holograms of a particle sample volume and then adding the resulting differences, a final hologram is constructed that contains the time evolution of the object trajectory free from spurious background interference effects.
Claims5
A method for tracking the trajectory in three-dimensions and in time of an object in a sample volume comprising the steps of:
- a) recording a time-spaced sequence of digital in-line holograms, generating a sequence of N holograms;
- b) subtracting from a first hologram a second hologram in each successive pair of the sequence of N holograms to generate N/2 difference holograms;
- c) summing the N/2 difference holograms to generate a summed hologram;
- and
- d) reconstructing images of the object at a plurality of depths into the sample volume representing the trajectory of the object.
A method for tracking the trajectory in three-dimensions and in time of an object in a sample volume comprising the steps of:
- a) recording a time-spaced sequence of digital in-line holograms, generating a sequence of N holograms;
- b) subtracting a first hologram from each of the remaining holograms of the sequence of N holograms to generate N−1 difference holograms;
- c) summing the N−1 difference holograms to generate a summed hologram;
- and
- d) reconstructing images of the object at a plurality of depths into the sample volume representing the trajectory of the object.
A method for tracking the trajectory in three-dimensions and in time of an object in a sample volume comprising the steps of:
- a) recording a time-spaced sequence of digital in-line holograms, generating a sequence of N holograms;
- b) subtracting a first hologram from each of the remaining holograms of the sequence of N holograms to generate N−1 difference holograms;
- c) reconstructing an image of the object at a depth into the sample volume for each of the N−1 difference holograms, generating N−1 subject images;
- and
- d) combining the N−1 subject images to generate a 3-D image representing the trajectory of the object.
A method for tracking the trajectory in three-dimensions and in time of a plurality of objects in a sample volume comprising the steps of:
- a) recording a digital in-line hologram as a first series of pixels in a buffer;
- b) for a subsequent time-spaced sequence of digital in-line holograms, recording each hologram represented as a second series of pixels by:
- subtracting each pixel in the second series of pixels from a corresponding pixel in the buffer, for a hologram having an even ordinal number in the sequence of digital in-line holograms and
- adding each pixel in the second series of pixels to a corresponding pixel in the buffer, for a hologram having an odd ordinal number in the sequence of digital in-line holograms;
- and
- c) reconstructing images of the object at a plurality of depths into the sample volume representing the trajectory of the object.
Dependencies are inferred from the claim text.