High speed, high resolution compositions, methods and kits for capillary electrophoresis
AssigneeApplera Corporation
InventorsKarl O. Voss, Aldrich N.K. Lau
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Abstract
The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
Claims56
A composition for separating analytes by capillary electrophoresis comprising:
- a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Daltons (Da) and 3,000,000 Da;
- and
- a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
- wherein the sieving component and the surface interaction component are the same or different;
- wherein the composition does not include a crosslinked polymer gel.
A composition for separating analytes by capillary electrophoresis comprising:
- a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and having a viscosity of less than 10,000 centipoise at 25° C.;
- a surface interaction component comprising poly(N,N-dimethylacrylamide);
- and
- a denaturant comprising urea;
- wherein the composition does not include a crosslinked polymer gel.
A capillary electrophoresis element comprising:
- an uncoated capillary;
- a composition for separating analytes located within the uncoated capillary, the composition comprising:
- a sieving component comprising a uncrosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and having a viscosity of less than 10,000 centipoise at 25° C.;
- and a surface interaction component;
- wherein the surface interaction component comprises a solution of one or more non-crosslinked polymers;
- and
- wherein the capillary electrophoresis element does not include a crosslinked polymeric gel.
A method for separating analytes by capillary electrophoresis comprising:
- separating the analytes by capillary electrophoresis in a composition comprising:
- a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da;
- and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of C, to C3 alkyl, halo-substituted C, to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
- wherein the sieving component and the surface interaction component are the same or different;
- and wherein the composition does not include a crosslinked polymer gel.
A method for separating analytes by capillary electrophoresis comprising:
- inserting into an uncoated capillary having a first end and a second end a composition comprising a sieving component comprising an uncrosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
- wherein the sieving component and the surface interaction component are the same or different;
- wherein the composition does not include a crosslinked polymer gel;
- loading a sample of different sized analytes in the capillary;
- and
- applying an electrical field between the first and second ends of the capillary so that the different sized analytes in the sample migrate through the capillary, thereby separating the analytes.
A kit for separating analytes by capillary electrophoresis comprising a composition comprising:
- a sieving component comprising an uncrosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and having a viscosity of less than 1000 centipoise;
- and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
- wherein the sieving component and the surface interaction component are the same or different;
- wherein the composition does not include a crosslinking agent.
A kit for separating analytes by capillary electrophoresis comprising a composition comprising a sieving component comprising an non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Da and 3,000,000 Da and a viscosity of less than 1000 centipoise at 25° C., wherein the composition does not include a crosslinked polymer gel.
A composition for separating analytes by capillary electrophoresis comprising:
- a sieving component comprising a non-crosslinked acrylamide polymer having a molecular weight between about 1,000,000 Daltons (Da) and 3,000,000 Da, wherein the composition does not include a crosslinked polymer gel.
Dependencies are inferred from the claim text.