Miniature device for separating and isolation biological objects and uses thereof
OwnerAlexandra Fuchs, Patrice Caillat +2inventors
InventorsAlexandra Fuchs, Patrice Caillat, Daniel Dupret, Fabrice Lefevre
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Abstract
The invention concerns a miniature device for separating and isolating biological objects, the use of said device for isolating, separating, culturing and/or analysing biological objects and a method for separating and isolating biological objects using said device.
Claims36
A miniature device for separating and/or isolating biological objects, having at least one first electrode integrated with the device, consisting of a structure provided with an array of reaction microcuvettes, each microcuvette having a bottom consisting of a reception zone, characterized in that said bottom is devoid of holes and the maximum surface area of said bottom of each microcuvette is defined so as to isolate a single biological object, said structure being connected to a supply circuit in order to create a potential difference between said first electrode and at least one second electrode integrated with or external to the device.
The device as claimed in any one of the preceding claims, characterized in that the array of reaction microcuvettes is surmounted at least partly by one or more layers of insulating materials and/or an attached grid of biocompatible plastic, so as to form an array of microreservoirs.
The device as claimed in any one of the preceding claims, characterized in that one face of the first electrode integrated with the device constitutes the bottom of the microcuvettes.
The device as claimed in any one of the preceding claims, characterized in that it includes a plurality of said first electrodes electrically insulated from one another.
The device as claimed in any one of the preceding claims, characterized in that the second electrode is integrated with the device, and in that said electrode is deposited on a first layer of insulating material and lies in a plane separated from the bottom of said microcuvettes.
The device as claimed in any one of the preceding claims, characterized in that it has at least one third electrode integrated with the device, a second layer of insulating material being interposed between the second and third electrodes.
The device as claimed in any one of the preceding claims, characterized in that it is equipped with an integrated circuit for multiplexing at least some of said electrodes.
The device as claimed in any one of the preceding claims, characterized in that a reagent capable of fixing the biological object to be isolated is fixed on at least one part of a reception zone of the reaction microcuvettes.
The device as claimed in any one of the preceding claims, characterized in that it is equipped with a closing means.
The use of at least one miniature device as claimed in any one of the preceding claims, for the isolation, separation, culture and/or analysis of biological objects.
Dependencies are inferred from the claim text.