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US 2004/0168915 A1Published application
Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis
AssigneeCombisep, Inc.
InventorsYan He, Ho-Ming Pang, Siquan Luo, Futian Han
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Abstract
Disclosed is a two-dimensional protein separation method. It makes separating a protein sample by chromatofocusing into a plurality of aliquots, and then loading each aliquot into a separate capillary tube; and separating each aliquot by multiplexed capillary electrophoresis to produce a two-dimensional array of separated proteins. A preferred integrated buffer for this system is also disclosed.
Claims4
A two-dimensional protein separation method comprising:
- obtaining a test sample suspected of containing a plurality of proteins;
- treating the sample with a chromatofocusing system to produce a first separated protein sample;
- and thereafter
- treating at least a portion of the first separated protein test sample with multiplexed gel electrophoresis to produce a second separated protein sample;
- and
- determining from the combination of chromatofocusing and multiplexed capillary electrophoresis data the protein content of the sample.
3 more elements
A protein separation apparatus comprising:
- a chromatofocusing system for producing separated protein samples at an output;
- a multiplexed capillary electrophoresis system having inputs for receiving protein samples;
- and
- wherein the output of the chromatofocusing system is the input to the multiplexed capillary electrophoresis system such that the separated protein samples are further separated by the multiplexed capillary electrophoresis system.
1 more element
Dependencies are inferred from the claim text.