Transcriptional activator nucleic acids, polypeptides and methods of use thereof
AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsKeith S. Lowe, William J. Gordon-Kamm, Theodore M. Klein, Sonriza Rasco-Gaunt, Xifan Sun, George J. Hoerster
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Abstract
The invention provides isolated nucleic acids and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
Claims39
A method for enhancing tissue culture response in a plant cell comprising introducing into the plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide.
A method for inducing somatic embryogenesis in a plant cell comprising introducing into a plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide, wherein the plant cell is transformed and grown to produce a transformed embryo and wherein the plant cell is not an Arabidopsis cell.
A method for positive selection of a transformed cell comprising introducing into a plant cell at least one LEC1 polynucleotide or at least one LEC1 polypeptide and growing the transformed plant cell, wherein the plant cell is transformed and grown under conditions sufficient to induce embryogenesis to provide a positive selection means.
A method for inducing apomixis in a plant cell comprising introducing into a responsive plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide and growing the plant cell, wherein the introducing and growing is done under conditions sufficient to produce a transformed somatic embryo.
A method for increasing transformation efficiency comprising introducing at least one LEC1 polypeptide or at least one LEC1 polynucleotide into a plant cell.
A method for increasing recovery of regenerated plants comprising introducing into a plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide and growing the plant cell.
Dependencies are inferred from the claim text.