Plant gene expression system for processing, targeting and accumulating foreign proteins in transgenic seeds
OwnerLiwen Jiang, Samuel Sai Ming Suninventors
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Abstract
A DNA construct generates and directs the processing, targeting and stable accumulation of target proteins in transgenic plant seeds. A method for constructing transgenic plants provides a general strategy in which unique transmembrane domain and cytoplasmic tail sequences are used as anchors for delivering recombinant target proteins via distinct vesicular transport pathways to specific vacuolar compartments, thus enabling stable accumulation of foreign target proteins in transgenic plants. A plant gene expression system has flexibility to allow the target proteins to bypass or subject to plants Golgi-specific post-translational modifications.
Claims29
A DNA construct to generate and direct the processing, targeting and stably accumulating of target proteins in transgenic plant seeds, comprising:
- a promoter sequence capable of directing expression in cells of the plant seeds;
- a first DNA sequence encoding the target proteins;
- a second DNA sequence having a transmembrane domain sequence and a cytoplasmic tail sequence which serve as anchors for delivering the target proteins to subcompartments of protein storage vacuoles of the cells;
- and
- a third DNA sequence functioning as a termination region in the plant.
Dependencies are inferred from the claim text.