Nucleic acids encoding anthelmintic agents and plants made therefrom
AssigneeDivergence, Inc.
InventorsMichelle L. Verbsky, Catherine Baublite, Deryck J. Williams, Andrew P. Kloek, Jennifer A. Davila-Aponte, Michelle Coutu Hresko, Merry B. McLaird, Rodolfo Zentella
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Abstract
The present invention provides DNA constructs, transgenic plants containing such constructs, and methods of making the plants. The DNA constructs encode a polypeptide that when expressed results in the production of fatty acid compounds having anthelmintic activity. Transgenic plants expressing such a polypeptide can exhibit enhanced resistance to plant parasitic nematodes, particularly when expressed in vegetative tissues.
Claims93
A transgenic plant containing at least one DNA construct, said construct comprising:
- a) a nucleic acid encoding a polypeptide effective for catalysing the conversion of a substrate to a C16, C18, or C20 monounsaturated fatty acid product selected from the group consisting of:
- 5
- wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, wherein both R1 and R2 are hydroxyl, one of R1 and R2 is hydroxyl and the other is hydrogen, or one of R1 and R2 is keto and the other is hydrogen, and wherein R3 is C2, C4, or C6 alkyl;
- and
- 6
- wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, and wherein R3 is C2, C4, or C6 alkyl;
- and
- (b) a regulatory element operably linked to said nucleic acid encoding said polypeptide, wherein said regulatory element confers expression in a vegetative tissue of said plant.
An isolated nucleic acid comprising the nucleotide sequence set forth in any one of SEQ ID NOS:
- 3 to 12 or 25 to 33.
A recombinant nucleic acid construct comprising at least one regulatory element that confers expression in a vegetative tissue of a plant, said regulatory element operably linked to a nucleic acid having the nucleotide sequence set forth in any one of SEQ ID NOS:
- 3 to 12 or 25 to 33.
A transgenic plant harboring a DNA construct comprising a nucleic acid encoding a fatty acid epoxygenase polypeptide or a fatty acid hydroxylase polypeptide operably linked to a regulatory element conferring expression of said polypeptide in a vegetative tissue of said plant.
Dependencies are inferred from the claim text.