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AssigneesPioneer Hi-Bred International, Inc., CuraGen Corporation
InventorsJonathan P. Duvick, Jacob T. Gilliam, Joyce R. Maddox, Oswald R. Crasta, Otto Folkerts
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The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
An isolated polynucleotide comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A recombinant expression cassette comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A vector comprising a recombinant expression cassette comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A host cell comprising a recombinant expression cassette comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A transformed plant comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A plant seed comprising a polynucleotide having at least 95% sequence identity to a polynucleotide selected from the group consisting of:
A method of reducing pathogenicity of a fungus producing fumonisin or a structurally related mycotoxin, comprising:
A method of making an APAO enzyme comprising the steps of:
Dependencies are inferred from the claim text.