Process for purifying pravastatin sodium from a fermentation broth
AssigneeChunghwa Chemical Synthesis & Biotech Co. Ltd.
InventorsJing-Chen Mai, Chiou-Jour Laih, Yu-Chin Chiang
Life of this patent
Estimating the term…
Abstract
A process for purifying pravastatin sodium comprising the steps of: a. clarifying a fermentation broth containing pravastatin sodium to obtain a clear solution; and adjusting the clear solution to be basic having pH value ranging from pH 10˜13; b. adsorbing the pravastatin sodium with non-ionic resin; eluting the pravastatin sodium by water-soluble organic solvent; and forming a concentrate of pravastatin sodium; c. treating the concentrate with water-soluble anti-solvent or inorganic salt to form a precipitate of pravastatin sodium; and d. recrystallizing the precipitate for making pravastatin sodium crystal with high purity and high yield.
Claims6
A process for stably purifying pravastatin sodium comprising the steps of:
- a. clarifying a fermentation broth containing pravastatin sodium by a step selected from centrifugation and filtration to obtain a clear solution containing the pravastatin sodium;
- and adjusting the clear solution to be basic having pH value ranging from pH 10˜13;
- b. adsorbing the pravastatin sodium with non-ionic resin which is then washed with water;
- eluting the pravastatin sodium as adsorbed on the resin by water-soluble organic solvent to obtain pravastatin sodium fraction;
- and concentrating the fraction to be pravastatin sodium concentrate;
- c. treating the concentrate to form a precipitate of pravastatin sodium;
- and
- d. recrystallizing the precipitate for making pravastatin sodium crystal with high purity and high yield.
Dependencies are inferred from the claim text.