Pharmacological agents and methods of treatment that inactivate pathogenic prokaryotic and eukaryotic cells and viruses by attacking highly conserved domains in structural metalloprotien and metalloenzyme targets

A method for inactivating a metalloprotein or metalloenzyme of a pathogenic virus, pathogenic prokaryotic organisms or pathogenic eukaryotic cells, wherein the said protein comprises an amino acid sequence structure which chelates a zinc ion or a transition metal ion, said method comprising the step of contacting intravirally or intracellularly the said zinc ion or transition metal ion bound to the chelating protein structure with a chelating compound which dissociates the metal ion protein complex selected from the group consisting of the following compounds:

• 2-furoic acid;
• 2-furoic acid hydrazide;
• Tetrahydro-2-furoic acid;
• 3,5-dibromo-2-furoic acid;
• 3,4,5-tribromo-2-furoic acid;
• 2,5-dimethoxytetrahydro-2-furoic acid hydrazine;
• 5-[2-Chloro-5-(trifluoromethyl)phenyl]-2-furoic acid;
• 5-[3-(trifluoromethyl)phenyl]-2-furoic acid;
• 5-(2-Nitrophenyl)-2-furoic acid;
• 5-(3-Nitrophenyl)-2-furoic acid;
• 5-(4-Chloro-2-nitrophenyl)-2-furoic acid;
• 5-(4-Chlorophenyl)-2-furoic acid;
• 5-(4-Methyl-2-nitrophenyl)-2-furoic acid;
• 5-(4-Nitrophenyl)-2-furoic acid;
• 5-Bromo-2-furoic acid;
• 5-Chloro-2-furoic acid;
• 5-Nitro-2-furoic acid;
• 5-Nitrofuran-2-carboxylic acid;
• 2-thiophenecarboxylic acid;
• 3,4,5-trichloro-2-thiophenecarboxylic acid;
• 2-thiophenecarboxylic acid hydrazide;
• 2-thiophenecarboxylic acid hydrazone;
• 2-thiophenecarboxylic acid thiosemicarbazone;
• 3-methyl-2-thiophenecarboxylic acid;
• 5-bromo-2-thiophenecarboxylic acid hydrazone;
• 5-methyl-2-thiophenecarboxylic acid;
• 5-chloro-2-thiophenecarboxylic acid;
• and 5-chloro-2-thiophenecarboxylic acid hydrazone;

A compound having the formula:

• 14
• Wherein R1, which can be singly or multiply substituted in any position of the thiophene ring not already substituted by R2, is selected from the group consisting of hydrogen, alkyl and substituted alkyl wherein the alkyl portion is from 1 to 21 carbon atoms, inclusive, and isomeric forms thereof, cycloalkyls and substituted cycloalkyl, substituted oxygen, substituted nitrogen, halogen, phenyl, and substituted phenyl, —(CH2)n—OH, —-(CH2)—NR3R4, and isomeric forms thereof, wherein n is an integer of from 1 to 21, inclusive, R3 and R4 are H or alkyl of from 1 to 21 carbon atoms, inclusive, and isomeric forms thereof;
• wherein R2, which can be singly or multiply substituted in any position of the thiophene ring not already substituted by R1, is
• 15
• and X is the amino function of a compound selected from the group consisting of 2-hydrazine, 2-hydrazone, or 2-thiosemicarbazone.

A composition comprising at least two compounds from the following compound groups represented by the formula:

• 17
• Wherein R1, which can be singly or multiply substituted in any position of the thiophene ring not already substituted by R2, is selected from the group consisting of hydrogen, alkyl and substituted alkyl wherein the alkyl portion is from 1 to 21 carbon atoms, inclusive, and isomeric forms thereof, cycloalkyls and substituted cycloalkyl, substituted oxygen, substituted nitrogen, halogen, phenyl, and substituted phenyl, —(CH2)n—OH, —(CH2)n—NR3R4, and isomeric forms thereof, wherein n is an integer of from 1 to 21, inclusive, R3 and R4 are H or all of from 1 to 21 carbon atoms, inclusive, and isomeric forms thereof, wherein R2, which can be singly or multiply substituted in any position of the thiophene ring not already substituted by R1, is
• 18
• and X is the amino function of a compound selected from the group consisting of 2-hydrazine, 2-hydrazone, or 2-thiosemicarbazone.

A composition comprising at least two compounds from the following compound groups:

• 1. A compound comprising hydrazines of furoic acid having the formula —C—NH—NH—R, where —C is attached to position 2 of the thiophene ring;
• 2. A compound comprising hydrazones of furoic acid having the formula —CH═N—NH—C═O—, where —C is attached to position 2 of the thiophene ring;
• 3. A compound comprising thiosemicarbazones of furoic acid having the formula —CH═N—NH—C═S—, where C is in position 2 of the thiophene ring;
• 4. A compound comprising halogenated furoic acid and derivatives thereof where the halogen is selected from the group consisting of F,I,Br, and Cl;
• The compounds of 1 to 4 coordinately complexed to cupric ions or to ferric ions, or to toxic metals such as platinum.

A composition comprising at least two compounds from the following compound groups:

• 1. A compound comprising hydrazines of 2-thiophenecarboxylic acid having the formula —C—NH—NH—R, where —C is attached to position 2 of the thiophene ring;
• 2. A compound comprising hydrazones of 2-thiophenecarboxylic acid having the formula —CH═N—N—H—C═O—, where —C is attached to position 2 of the thiophene ring;
• 3. A compound comprising thiosemicarbazones of 2-thiophenecarboxylic acid having the formula —CH═N—NH—C═S—, where C is in position 2 of the thiophene ring;
• 4. A compound comprising halogenated 2-thiophenecarboxylic acid, and derivatives thereof where the halogen is selected from the group consisting of F,I,Br, and Cl;
• The compounds of 1 to 4 coordinately complexed to cupric ions or to ferric ions, or to toxic metals such as platinum.

A composition comprising at least two compounds from the following compound groups:

• 1. A compound comprising hydrazines of furoic acid having the formula —C—NH—NH—R, where —C is attached to position 2 of the thiophene ring and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 2. A compound comprising hydrazones of furoic acid having the formula —CH═N—NH—C═O—, where —C is attached to position 2 of the thiophene ring;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 3. A compound comprising thiosemicarbazones of furoic acid having the formula —CH═N—NH—C═S—, where C is in position 2 of the thiophene ring;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 4. A compound comprising halogenated furoic acid and derivatives thereof where the halogen is selected from the group consisting of F,I,Br, and Cl;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• The compounds of 1 to 4 coordinately complexed to cupric ions or to ferric ions, or to toxic metals such as platinum.

A composition comprising at least two compounds from the following compound groups:

• 1. A compound comprising hydrazines of 2-thiophenecarboxylic acid having the formula —C—NH—NH—R, where —C is attached to position 2 of the thiophene ring and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 2. A compound comprising hydrazones of 2-thiophenecarboxylic acid having the formula —CH═N—NH—C═O—, where —C is attached to position 2 of the thiophene ring;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 3. A compound comprising thiosemicarbazones of 2-thiophenecarboxylic acid having the formula —CH═N—N—NH—C═S—, where C is in position 2 of the thiophene ring;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• 4. A compound comprising halogenated 2-thiophenecarboxylic acid, and derivatives thereof where the halogen is selected from the group consisting of F,I,Br, and Cl;
• and in which one or more of the ring residues in positions 3 or 4 have been replaced by a fatty acid side chain of 2 to 21 carbons.
• The compounds of 1 to 4 coordinately complexed to cupric ions or to ferric ions, or to toxic metals such as platinum.