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OwnerAchim Aigner, Frank Czubayko +2inventors
InventorsAchim Aigner, Frank Czubayko, Thomas Kissel, Dagmar Fischer
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The invention relates to the complexation of RNA with optionally modified polyethylenimines for extracellular and intracellularin vitro and in vivo stabiliztion thereof and for cellular introduction of the complexed RNA in cells. The invention also relates to chemically modified or non-modified, natural or synthetic ribozymes complexes and polyethylenimine (PEI) having any chain length, molar mass and degree of branching, which is chemically modified or non-chemically modified. The invention further relates to the production of complexes and to the use thereof in the protection of RNA against enzymatic and non-enzymatic degradation and their reception in vitro or in vivo in cells. The biological activity of the ribozymes remains intact.
A method of using complexes of RNA molecules with polyethylene imines (PEI) which are optionally modified with hydrophilic polymers coupled thereto for protecting RNA from enzymatic or non-enzymatic, intra- or extracellular decomposition.
A complex comprising a ribozyme of a chain length of 20-600 nucleotides, preferably a hammerhead ribozyme, and polyethylene imine (PEI) which is optionally modified with hydrophilic polymers coupled thereto.
Dependencies are inferred from the claim text.