Novel immunogenic compositions for the prevention and treatment of meningococcal disease
AssigneeWyeth Holdings Corporation
InventorsGary W. Zlotnick, Leah Diane Fletcher, John Erwin Farley, Liesel A. Bernfield, Robert J. Zagursky, Benjamin J. Metcalf
Life of this patent
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Abstract
The present invention relates to Neisseria ORF2086 proteins, crossreactive immunogenic proteins which can be isolated from nesserial strains or prepared recombinantly, including immunogenic portions thereof, biological equivalents thereof, antibodies that immunospecifically bind to the foregoing and nucleic acid sequences encoding each of the foregoing, as well as the use of same in immunogenic compositions that are effective against infection by Neisseria meningitidis serogroup B.
Claims107
A composition comprising:
- at least one immunogenic protein or polypeptide comprising any of even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition comprising:
- at least one antibody that immunospecifically binds with at least one protein comprising any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition comprising:
- at least one a polynucleotide comprising the nucleic acid sequence of any of odd numbered SEQ ID NOS:1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A composition comprising:
- a vector comprising at least one polynucleotide comprising the nucleic acid sequence of any of odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A composition comprising:
- a vector comprising at least one polynucleotide that encodes at least one of the polypeptides comprising the amino acid sequence of any of even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition comprising:
- a host cell transformed/transfected or infected with a vector, said vector comprising at least one polynucleotide encoding a protein comprising the amino acid sequence of any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition prepared by a process comprising:
- expressing in a host cell a nucleic acid sequence encoding a protein comprising any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition prepared by a process comprising:
- isolating and purifying from Neisseria species at least one protein comprising any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition prepared by a process comprising:
- inserting into a vector at least one isolated protein comprising the amino acid sequence of any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition prepared by a process comprising:
- isolating and purifying from Neisseria species at least one polynucleotide that encodes at least one of the polypeptides comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A composition prepared by a process comprising:
- inserting into a vector at least one isolated polynucleotide that encodes any of the polypeptides comprising any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A method of preparing an antibody composition comprising:
- recovering antibodies from an animal after introducing into the animal a composition comprising a protein comprising the amino acid sequence of any of the even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A method of inducing an immune response in a mammal comprising:
- administering to the mammal an effective amount of a composition comprising:
- (a) a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) a polynucleotide comprising the nucleic acid sequence of any of the odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A method of preventing or treating bacterial meningitis in a mammal comprising:
- administering to the mammal an effective amount of a composition comprising:
- (a) a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) a polynucleotide comprising the nucleic acid sequence of any of the odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A method of preventing or treating bacterial meningitis in a mammal comprising:
- administering to the mammal an effective amount of an antibody composition comprising antibodies that immunospecifically bind with a protein, immunogenic portion or biological equivalent comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246.
A method of preparing a composition comprising:
- expressing in a host cell a nucleic acid sequence encoding any of:
- (a) at least one protein comprising the amino acid sequence of any of even numbered SEQ ID NOS:2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) at least one protein encoded by a polynucleotide comprising the nucleic acid sequence of any of odd numbered SEQ ID NOS:1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A method of preparing a composition comprising:
- isolating and purifying from Neisseria species any of:
- (a) at least one protein comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) at least one protein encoded by a polynucleotide comprising the nucleic acid sequence of any of the odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A method of preparing an antibody composition comprising:
- recovering antibodies from an animal after introducing into the animal a composition comprising:
- (a) at least one protein comprising the amino acid sequence of any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) at least one protein encoded by a polynucleotide comprising the nucleic acid sequence of any of the odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A transformed/transfected or infected cell line comprising:
- a recombinant cell that expresses a nucleic acid sequence comprising:
- (a) at least one polynucleotide that encodes a protein comprising any of the even numbered SEQ ID NOS:
- 2-24, 32-48, 62-72, 80-90, 122-126, 146-150, 158-168, 176-180 or 236-246;
- or
- (b) at least one polynucleotide comprising the nucleic acid sequence of any of the odd numbered SEQ ID NOS:
- 1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245.
A method for identifying an immunogenic protein comprising:
- detecting a polynucleotide comprising the nucleic acid sequence of any of odd numbered SEQ ID NOS:1-23, 31-47, 61-71, 79-89, 121-125, 145-149, 157-167, 175-179 or 235-245 in a sample tested against an ORF2086 nucleic acid probe.
Dependencies are inferred from the claim text.