Adaptive interferometric multi-analyte high-speed biosensor
OwnerDavid D. Nolte, Fred E. Regnier +1inventors
InventorsDavid D. Nolte, Fred E. Regnier, Leilei Peng
Life of this patent
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Abstract
A device for identifying analytes in a biological sample, including a substrate configured to bind the analyte and a detection system to determine the presence or absence of the analyte in the biological sample.
Claims44
A method for detecting an analyte within a sample, comprising the steps of:
- providing a substrate having a plurality of spaced-apart targets configured to bind the analyte;
- sequentially directing an optical signal at each of the plurality of spaced-apart targets;
- and
- detecting the presence of the analyte with an interferometer.
A device for identifying a first analyte of a group of analytes in a biological sample, including:
- a substrate including a first plurality of spaced-apart regions configured to bind the first analyte;
- an optical source configured to generate a probe beam which illuminates the first plurality of spaced-apart regions in a sequential manner and interacts with the substrate to form a signal beam;
- an interferometer configured to combine with an adaptive optical element a reference beam and the signal beam, the combination of the reference beam and the signal beam generating an output beam;
- and
- a detector configured to detect the presence or absence of the first analyte based upon the output beam.
A substrate configured to bind a plurality of analytes and for use with a detection system configured to illuminate the substrate with a probe beam and to determine the presence of one of the analytes on the substrate based on the characteristics of a probe beam subsequent to interacting with the substrate, the substrate comprising:
- a circular track including a plurality of spaced-apart targets configured to bind to one analyte, the plurality of spaced-apart targets being separated by a plurality of regions configured to not bind the one analyte;
- and
- wherein the plurality of spaced-apart targets and the plurality of regions together are configured to alter the characteristics of the probe beam to produce a first signal when the one analyte is bound to the plurality of spaced-apart targets and to alter the characteristics of the probe beam to produce a second signal when the one analyte is not bound to the plurality of spaced apart targets.
A device for identifying an analyte in a biological sample, including:
- a substrate including a first region configured to bind the analyte upon introduction of the biological sample to the substrate and a second region configured to not bind the analyte;
- a motor configured to spin the substrate;
- a laser including a beam which is directed at the substrate such that the beam interacts with the first region and the second region as the substrate spins;
- and
- a sensor configured to detect a portion of the beam subsequent to the beam interacting with the substrate, the sensor indicating the presence of the analyte based on an interference characteristic of the beam subsequent to the beam interacting with the substrate, the beam having a first interference characteristic if the analyte is bound to the substrate and a second interference characteristic if the analyte is not bound to the substrate.
A device for identifying analytes in a biological sample, including:
- a substrate having a surface lying substantially in a first plane, a plurality of targets offset vertically from the substrate surface, each of the targets having a wall lying substantially in a second plane, one of the surface and the walls of the targets being configured to bind analytes present in the biological sample when the biological sample is introduced to the substrate;
- and
- a laser for sequentially directing a beam at each of the plurality of targets, the laser being positioned relative to the substrate such that when the beam is directed at a target an interference characteristic is produced.
A method for detecting the presence of an analyte in a sample, comprising the steps of:
- providing a substrate having a plurality of first regions configured to bind the analyte and a plurality of second regions configured to not bind the analyte;
- spinning the substrate;
- introducing the sample proximate the center of the substrate and continuing to spin the substrate such that an incubation time for the sample is generally diffusion limited;
- directing a laser beam at the substrate such that the laser interacts with the substrate;
- and
- determining the presence of the analyte based at least in part on a characteristics of laser beam subsequent to interaction with the substrate.
Dependencies are inferred from the claim text.