Vector constructs
AssigneeDevgen NV
InventorsGeert Plaetinck, Jean-pierre Renard, Thierry Bogaert
Life of this patent
Estimating the term…
Abstract
Improved vector constructs useful in the expression of double-stranded RNA are provided. The constructs are particularly useful for expression of double-stranded RNA in vitro and in vivo.
Claims28
A DNA construct comprising:
- a) a first promoter and
- b) a second promoter, in which the first and second promoter are in opposite orientation to each other and define:
- c) an inter-promoter region positioned downstream of the 3′ end of the first promoter and downstream of the 3′ end of the second promoter;
- and which DNA construct further comprises:
- d) at least one cloning site positioned in the inter-promoter region;
- and
- e) a first transcription terminator, positioned (as seen from the 3′ end of the first promoter) downstream of the first promoter and downstream of the at least one cloning site, wherein the first transcription terminator is operably linked to the first promoter.
A construct according to any one of the preceding claims wherein the first and the second promoter are identical.
A construct according to any one of the preceding claims wherein the cloning site comprises at least one restriction site.
A DNA construct according to any one of the preceding claims wherein the cloning site further comprises attR1 and attR2 recombination sequences.
A DNA construct according to any one of the preceding claims wherein the cloning site further comprises a ccdB nucleotide sequence.
A DNA according to any one of the preceding claims which further comprises:
- g) a DNA fragment inserted in the at least one cloning site.
A DNA construct according to any one of the preceding claims which is a plasmid or vector.
Use of the DNA construct according to any one of the preceding claims for the production of double-stranded RNA for RNA inhibition.
A bacterial strain harbouring the DNA construct according to any one of the preceding claims.
Dependencies are inferred from the claim text.