Back to results
US 2004/0166579 A1Published application
Cell culture media for mammalian cells
AssigneeUniversity of Pittsburgh
InventorGeoffrey D. Block
Life of this patent
Estimating the term…
Abstract
A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells.
Claims25
A chemically defined HBM culture medium for maintenance, differentiation, and long-term growth of mammalian hepatocytes, comprising:
- (a) a synthetic stock basal medium designed for mammalian cell culture;
- and
- (b) a hepatocyte cell growth promoting amount of components selected from among nicotinamide, amino acids, transferrin, hormones, dexamethasone, trace metals, and simple carbohydrate selected from the group consisting of D-glucose and D-galactose and any combination thereof.
A mammalian cell culture medium comprising the composition of HGM as defined in Tables I and II, wherein the stock basal media of Table I comprises a blended DMEM such that the final concentration of is D-glucose is preferably about 2.0 g/L and the amount of D-galactose is preferably about 2.0 g/L.
Dependencies are inferred from the claim text.