D-aminoacylase and gene encoding the same
AssigneeDaicel Chemical Industries, Ltd., a Japan corporation
InventorsKazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
Life of this patent
Estimating the term…
Abstract
The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.
Claims13
A polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) a polynucleotide encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2;
- (c) a polynucleotide hybridizing to a DNA comprising the nucleotide sequence set forth in SEQ ID NO:
- 1 under a stringent condition, wherein said polynucleotide encodes a polypeptide having the activity of a D-aminoacylase having the physicochemical properties of (i) and (ii) below;
- and
- (d) a polynucleotide encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 in which one or more amino acid are substituted, deleted, inserted, and/or added, wherein said polynucleotide encodes a polypeptide having the activity of a D-aminoacylase having the physicochemical properties of (i) and (ii) below
- (i) action:
- the enzyme acts on N-acetyl-D-amino acids to produce the corresponding D-amino acids and
- (ii) substrate specificity:
- the enzyme acts on N-acetyl-D-tryptophan, N-acetyl-D-phenylalanine, N-acetyl-D-valine, N-acetyl-D-leucine, and N-acetyl-D-methionine, but not on N-acetyl-L-tryptophan, N-acetyl-L-phenylalanine, N-acetyl-L-valine, N-acetyl-L-leucine, or N-acetyl-L-methionine.
A polynucleotide hybridizing to the polynucleotide set forth in SEQ ID NO:
- 1 or the complement thereof, wherein said polynucleotide comprises at least 15 nucleotides.
A method for producing D-amino acids, said method comprising contacting a polypeptide with N-acyl-DL-amino acid represented by the formula (I) or its salt:
- 3
- wherein R1 and R2 may be identical or different and each represents a hydrogen atom or a substituted or unsubstituted hydrocarbon group;
- R2 does not represent a hydrogen atom;
- and X is H, NH4, or a metal ion.
Dependencies are inferred from the claim text.