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AssigneeAmano Pharmaceutical Co., Ltd.
InventorsShotaro Yamaguchi, Akira Matsuura
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A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.
An enzyme which has an activity to deamidate amido groups in a protein.
An enzyme which has an activity to deamidate amido groups in a protein by directly acting upon the amido groups without cutting peptide bonds and without cross-linking a protein.
A polypeptide which comprises a polypeptide having an activity to deamidate amido groups in protein and having the amino acid sequence of Sequence No. 6 shown in the Sequence Listing wherein one or more of amino acid residues of the amino acid sequence may be modified by at least one of deletion, addition, insertion and substitution.
A polypeptide which has the amino acid sequence of Sequence No. 6 shown in the Sequence Listing.
A nucleotide which encodes a polypeptide having an activity to deamidate amido groups in protein.
A nucleotide which encodes a polypeptide having an activity to deamidate amido groups in a protein by directly acting upon the amido groups without cutting peptide bonds and without cross-linking a protein.
A nucleotide which comprises a nucleotide being selected from the following nucleotides (a) to (g) and encoding a polypeptide having an activity to deamidate amido groups in protein;
A nucleotide which comprises a nucleotide encoding a polypeptide having the amino acid sequence of Sequence No. 6 shown in the Sequence Listing.
A method for producing a novel enzyme, which comprises culturing a microorganism in a nutrient medium, thereby allowing said microorganism to produce a novel enzyme having an activity to deamidate amido groups in protein, and subsequently collecting said enzyme.
A method for producing a novel enzyme having an activity to deamidate amido groups bonded in protein, which comprises culturing a microorganism in a nutrient medium, thereby allowing the microorganism to produce a novel enzyme which has an activity to deamidate amido groups in protein by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein, and subsequently collecting said enzyme.
A method for modifying a protein and/or a peptide, which comprises allowing an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein to react with a protein and/or a peptide.
A composition for use in modification of a protein and/or a peptide, which comprises an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein, as the active ingredient.
A method for improving functionality of a plant or animal protein and/or a peptide, which comprises allowing an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein to act upon said protein and/or peptide.
A method for improving functionality of food containing a plant or animal protein and/or a peptide, which comprises allowing an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein to act upon said food.
A method for improving extraction efficiency of a plant or animal protein and/or a peptide from a crude material, which comprises allowing an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein to act upon a crude material containing said protein and/or peptide.
A method for controlling reaction of transglutaminase, which comprises using an enzyme having an activity to deamidate amido groups in protein and peptide by directly acting upon the groups without causing severing of peptide bond and cross-linking of protein.
A method for modifying a target protein or peptide, comprising allowing an enzyme, which is capable of deamidating amido groups in target proteins and peptides by directly acting upon said amido groups without cutting peptide bonds and without cross-linking said target proteins or peptides, to act on said target protein or peptide.
Dependencies are inferred from the claim text.