Screening method for apoptosis and necrosis
AssigneeAponetics Ltd.
InventorsThomas Harr, Alessandro Strebel, Peter Erb, Sinuhe Hahn
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Abstract
The present invention relates to a method for the determination of non-, anti-, or pro-apoptotic and necrotic conditions of cells, newly designed vectors coding for marker proteins, cell lines transfected with such vector, and a method to assay the non-, pro- or anti-apoptotic or necrotic activity of test compounds.
Claims25
A method for the determination of apoptotic and/or necrotic conditions of living test cells comprising monitoring changes in the signal of a marker protein in said cells.
A vector comprising a gene coding for a marker protein which is operably linked to one or more strong promoters, such as the hEF1-α promoter, the MoLV-LTR promoter or a combination of the CMV and the MoLV-LTR promoter.
A method to assay the non-, pro- or anti-apoptotic or necrotic activity of a test compound and/or of a physical stimulus in living test cells comprising monitoring the change in the signal of a marker protein in said cells.
Dependencies are inferred from the claim text.