Methods of detecting CD34 positive and negative hematopoietic stem cells in human samples
AssigneeSysmex Corporation
InventorsFu-Sheng Wang, Mark Dorfner, Kojiro Hirai, Yukio Hamaguchi
Life of this patent
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Abstract
Methods of detecting CD34 negative hematopoietic stem cells in a blood sample are described that include (a) removing CD34 positive cells from the blood sample thereby producing a modified blood sample; (b) extracting CD133 positive cells from the modified blood sample thereby producing a fraction comprising CD34 negative hematopoietic stem cells; and (c) analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
Claims24
A method of detecting CD34 negative hematopoietic stem cells in a blood sample, comprising:
- removing CD34 positive cells from the blood sample thereby producing a modified blood sample;
- extracting CD133 positive cells from the modified blood sample thereby producing a fraction comprising CD34 negative hematopoietic stem cells;
- and
- analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
A method of detecting CD34 negative hematopoietic stem cells in a blood sample, comprising:
- treating the blood sample with CD34 antibodies to produce a modified blood sample substantially depleted of CD34 positive cells;
- treating the modified blood sample with CD133 antibodies to obtain a fraction comprising CD133 positive cells and CD34 negative hematopoietic stem cells;
- and
- analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
A method of detecting CD34 hematopoietic stem cells in a blood sample, comprising:
- treating the blood sample with CD34 antibodies to produce a modified blood sample substantially depleted of CD34 positive cells and a fraction of the blood sample comprising CD34 positive cells removed from the blood sample;
- treating the modified blood sample with CD133 antibodies to obtain a fraction of the modified blood sample comprising CD133 positive cells and CD34 negative hematopoietic stem cells;
- analyzing the fraction of the blood sample with an automated hematology analyzer, thereby detecting the CD34 positive cells;
- and
- analyzing the fraction of the modified blood sample with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.
Dependencies are inferred from the claim text.