Reversed mammalian protein-protein interaction trap
OwnerSven Eyckerman, Jan Tavernier +1inventors
InventorsSven Eyckerman, Jan Tavernier, Joel Vandekerckhove
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Abstract
The present invention relates to a recombinant receptor, comprising a ligand-binding domain and a signaling domain that comprises a heterologous bait polypeptide, which receptor is inactivated by binding of a prey polypeptide to the heterologous bait peptide, either in presence or absence of a ligand binding to the ligand-binding domain. The receptor is activated by addition of a compound that disrupts the bait-prey interaction. The present invention also relates to a method of screening compounds that disrupt compound-compound-binding using the recombinant receptor.
Claims21
A recombinant receptor comprising:
- a ligand-binding domain and
- a domain that comprises a heterologous bait polypeptide,
- wherein the activation of said recombinant receptor is inhibited by binding of a prey polypeptide to said heterologous bait peptide.
A prey polypeptide comprising:
- a polypeptide that interacts with a bait polypeptide and
- a polypeptide comprising an inhibitor of activation of a receptor and/or a recruitment site for an inhibitor of activation of a receptor.
Dependencies are inferred from the claim text.