Optimization of synthetic catalysts by means of directed evolution
OwnerManfred T. Reetzinventor
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Abstract
The invention relates to a method for producing optimized catalysts which are optimized, during the course of said production method, in terms of a desired catalyst property or a plurality of properties. A synthetic metal-containing or metal-free catalyst is chemically linked to enzyme or protein mutants which are produced by means of molecular biology, and the catalysts created thereby are subjected to an optimization process using methods from molecular biology, or directed evolution of enzymes. The invention also relates to the catalysts which can be obtained by said method.
Claims13
A process for preparing a catalyst, which is a synthetic metal-containing or metal-free catalyst which is linked to an enzyme or protein and which is optimized in regard to one or more desired catalytic properties, wherein the process [lacuna] using methods of directed evolution to optimize the enzyme or the protein, linking a metal-containing or metal-free starting catalyst to the enzyme or protein, or modifying the catalyst with the enzyme or protein, and screening the resulting library of catalysts with a screening system which is appropriate in regard to the catalytic properties, and wherein, proceeding from a gene which encodes a wild-type enzyme or protein,
- (1) a library of mutated genes is generated by mutagenesis and from this,
- (2) a library of enzymes or proteins is generated by way of an expression system, and
- (3) the library of catalysts is generated by chemically linking the enzymes or the proteins to the metal-containing or metal-free synthetic starting catalyst, and
- (4) a screening system is used to select a catalyst which is appropriate in regard to the desired catalytic property.
Dependencies are inferred from the claim text.