Gene cloning
OwnerMichael G. Chaparian, Yiping Huanginventors
Life of this patent
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Abstract
There is provided a method of targeted cloning of genes and gene clusters by directly isolating the DNA of interest from a mixed population, thereby permitting the construction of a very targeted, highly enriched library. Also provided are several unique methods for cloning the genes provided by this method and the probes used in connection with this method.
Claims18
A method of targeted cloning and enrichment of genes and gene clusters by:
- directly isolating and subsequent cloning the targeted genes/cluster.
A method of isolating trimethoprim coding genes by directly isolating and subsequent cloning the trimethoprim coding gene.
Genes cloned by the method according to claim.
A method of providing degenerate cloning of an entire family of genes from a mixed DNA sample by directly isolating and subsequent cloning targeted genes/clusters.
Dependencies are inferred from the claim text.