Genetic polymorphisms associated with stenosis, methods of detection and uses thereof
AssigneeApplera Corporation
InventorsMichele Cargill, James J. Devlin, May M. Luke
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Abstract
The present invention is based on the discovery of genetic polymorphisms that are associated with stenosis. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection.
Claims24
A method for identifying an individual who has an altered risk for developing stenosis, comprising detecting a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences of SEQ ID NOS:1-284 and 569-26,130 in said individual's nucleic acids, wherein the presence of the SNP is correlated with an altered risk for stenosis in said individual.
An isolated nucleic acid molecule comprising at least 8 contiguous nucleotides wherein one of the nucleotides is a single nucleotide polymorphism (SNP) selected from any one of the nucleotide sequences in SEQ ID NOS:1-284 and 569-26,130, or a complement thereof.
An isolated nucleic acid molecule that encodes any one of the amino acid sequences in SEQ ID NOS:285-568.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOS:285-568.
An amplified polynucleotide containing a single nucleotide polymorphism (SNP) selected from any one of the nucleotide sequences of SEQ ID NOS:1-284 and 569-26,130, or a complement thereof, wherein the amplified polynucleotide is between about 16 and about 1,000 nucleotides in length.
An isolated polynucleotide which specifically hybridizes to a nucleic acid molecule containing a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences in SEQ ID NOS:1-284 and 569-26,130.
A method of detecting a single nucleotide polymorphism (SNP) in a nucleic acid molecule, comprising contacting a test sample with a reagent which specifically hybridizes to a SNP in any one of the nucleotide sequences of SEQ ID NOS:1-284 and 569-26,130 under stringent hybridization conditions, and detecting the formation of a hybridized duplex.
A method of detecting a variant polypeptide, comprising contacting a reagent with a variant polypeptide encoded by a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences of SEQ ID NOS:1-284 and 569-26,130 in a test sample, and detecting the binding of the reagent to the polypeptide.
Dependencies are inferred from the claim text.