Multilabeling mouse FISH assay for detecting structural and numerical chromosomal abnormalities
AssigneeThe Regents of the University of California
InventorsAndrew J. Wyrobek, Francesca S. Hill, Francesco Marchetti
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Abstract
Described herein is a method for detecting structural and numerical chromosome abnormalities in sperm cells. This invention can also be used to detect chromosome abnormalities resulting from exposure to factors such as chemicals, other materials, radiation and environmental conditions. This invention will allow for the wide spread screening of many different types of factors such as chemical, other materials, radiation and environmental conditions to determine whether they have the chromosome abnormality generating effects
Claims20
A method of detecting abnormalities in chromosomes of sperm cells comprising:
- staining target sperm cell chromosomal DNA with a nucleic acid probe set wherein the target sperm cell chromosomal DNA is stained by contacting the chromosomal DNA with said nucleic acid probe set which comprises a plurality of nucleotide segments complementary to the chromosomal DNA of interest, under conditions appropriate for hybridization of complementary DNA segments and wherein the chromosomal DNA is present in a sperm cell nucleus during the hybridization;
- and
- detecting hybridization between the sperm cell chromosomal DNA and said nucleic acid probe set, wherein the occurrence of hybridization shows the detection simultaneously of at least two types of chromosome abnormalities.
A method of detecting abnormalities in chromosomes of mouse sperm cells comprising:
- exposing one or more mice to at least one factor selected from chemicals, other materials, radiation and environmental conditions;
- harvesting sperm cells from said one or more mice;
- staining the chromosomal DNA of said sperm cells with a nucleic acid probe set wherein the chromosomal DNA of said sperm cells is stained by contacting the chromosomal DNA with said nucleic acid probe set which comprises a plurality of nucleotide segments complementary to the chromosomal DNA of interest, under conditions appropriate for hybridization of complementary DNA segments and wherein the chromosomal DNA is present in mice sperm cell nuclei during the hybridization;
- and
- detecting hybridization between the chromosomal DNA in said sperm cells and said nucleic acid probe set, wherein the occurrence of hybridization shows the detection simultaneously of at least two types of chromosome abnormalities.
A method of detecting abnormalities in chromosomes of cells comprising:
- staining target cell chromosomal DNA with a nucleic acid probe set wherein the target cell chromosomal DNA is stained by contacting the chromosomal DNA with said nucleic acid probe set which comprises a plurality of nucleotide segments complementary to the chromosomal DNA of interest, under conditions appropriate for hybridization of complementary DNA segments and wherein the chromosomal DNA is present in a cell nucleus during the hybridization;
- and
- detecting hybridization between the cell chromosomal DNA and said nucleic acid probe set, wherein the occurrence of hybridization shows the detection simultaneously of at least two types of chromosome abnormalities.
A method of detecting abnormalities in chromosomes 2 and 8 of mouse sperm cells comprising:
- staining chromosomes 2 and 8 of target mouse sperm cells with a nucleic acid probe set wherein the DNA of chromosomes 2 and 8 are stained by contacting said DNA with said nucleic acid probe set which comprises a plurality of nucleotide segments complementary to the chromosomal DNA of interest in chromosomes 2 and 8, under conditions appropriate for hybridization of complementary DNA segments and wherein the chromosomal DNA is present in chromosomes 2 and 8 of a mouse sperm cell nucleus during the hybridization;
- and
- detecting hybridization between the mouse sperm cell DNA of chromosomes 2 and 8 and said nucleic acid probe set, wherein the occurrence of hybridization shows the detection simultaneously of at least two types of chromosome abnormalities.
Dependencies are inferred from the claim text.