Isolated CEL II endonuclease
AssigneeTransgenomic, Inc.
InventorsGary F. Gerard, Harini Shandilya, Peter Qiu, James M. D'Alessio
Life of this patent
Estimating the term…
Abstract
The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided.
Claims12
A purified and isolated CEL II or CEL I endonuclease protein.
A method for separating CEL I and CEL II in a mixture comprising subjecting a mixture containing CEL I and CEL II to heparin affinity chromatography so that CEL I and CEL II are separated.
A composition comprising isolated CEL II endonuclease.
A method for detecting the presence of mismatches in double-stranded DNA comprising contacting a sample containing double-stranded DNA with an isolated CEL II endonuclease, separating the product of the CEL II endonuclease digestion, and detecting said product wherein an increase in the number of DNA fragments generated in the presence of the CEL II endonuclease is indicative of a mismatch in said DNA.
A kit for detecting the presence of mismatches in double-stranded DNA comprising an isolated CEL II endonuclease enzyme.
Dependencies are inferred from the claim text.