Analytical platform and detection method with the analytes to be determined in a sample as immobilized specific binding partners, optionally after fractionation of said sample

Abstract

The present invention is related to an analytical platform and a method performed therewith for the analysis of multiple samples for analytes which are contained therein and are of biological relevance as binding partners in specific binding reactions, wherein said samples or fractions of said samples, with the analytes to be determined contained therein, as a first plurality of specific binding partners, are deposited directly or after additional dilutions of said samples or fractions in discrete measurement areas in at least one one- or two-dimensional array of measurement areas on an evanescent field sensor platform as a solid support, different samples or fractions or different dilutions of samples or fractions being arranged in different discrete measurement areas, one or more tracer compounds as a second plurality of specific binding partners, for the specific determination of one or more analytes out of the first plurality of specific binding partners contained in the samples, are brought into contact with the samples or their fractions or dilutions deposited in said discrete measurement areas in a single step or multiple steps of a specific binding reaction, changes in opto-electronic signals, resulting from the binding of tracer compounds to analytes contained in discrete measurement areas in the evanescent field of the evanescent field sensor platform are measured laterally resolved, and the presence of the analytes to be specifically detected is determined qualititatively and/or quantitatively from the relative amount of the changes in said opto-electronic signals from the corresponding measurement areas.

Dependencies are inferred from the claim text.