Loading patent…
OwnerYoshihide Hayashizakiinventor
Estimating the term…
A linker or population of linkers comprising an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides comprising said linker or population of linkers and a target first strand polynucleotide bound to said linker. A method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. Provided is a linker instead of G tailing, which can be utilized in a method of preparing a cDNA library, and a method of preparing a cDNA library using said linker.
A linker or a population of linkers comprising an oligonucleotide fixed portion and an oligonucleotide variable portion, the variable portion being represented as Formula (N)n wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1, and if n is an integer equal to or higher than 2, the nucleotides (N) may be the same or different from each other, and Formula (N)n comprises at least one G.
A double strand oligonucleotide linker or a population of linkers, comprising
A method of marking a polynucleotide library and distinguishing said library, comprising the step of providing a population of linkers, comprising a fixed portion and a variable portion, wherein the fixed portion comprises at least one marker indicating a specific or defined tissue or species, and selecting and separating said library by said specific or defined marker.
A method of preparing a linker-polynucleotide product comprising a linker and a single strand polynucleotide, comprising annealing the variable portion of the linker according to the present invention to the target first strand polynucleotide.
(New) A linker or a population of linkers comprising an oligonucleotide fixed portion and an oligonucleotide variable portion, the variable portion being represented as Formula (N)n wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1, and if n is an integer equal to or higher than 2, the nucleotides (N) may be the same or different from each other, wherein the fixed portion has one or more sequences of recombinational sites, RNA polymerase promoter sites, markers or tags.
Dependencies are inferred from the claim text.