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US 2004/0166492 A1Published application
Quantitative detection of dekkera and saccharomyces
OwnerStacia R. Engel, Nancy A. Irelaninventors
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Abstract
DNA sequences are provided which are useful as hybridization probes for the real-time quantitative detection of Dekkera and Saccharomyces species.
Claims12
An oligonucleotide probe for identification of a fermentation-related microorganism, wherein said probe or its complement specifically binds to at least a portion of the ITS region of SEQ ID NOS:
- 3, 4 or 5 or which specifically binds to at least a portion of the ITS region of a fermentation-related organism selected from the group consisting of Dekkera bruxellensis, Saccharomyces bayanus, and Saccharomyces cerevisiae wherein said probe or its complement is selected from the group consisting of probes which contain at least 20 contiguous nucleotide bases from one of SEQ ID NOS:
- 1 or 2, probes which contain at least 20 contiguous nucleotide bases from one of SEQ ID NOS:
- 1 or 2 contiguous with 1 to 15 flanking nucleotide bases in the 5′ and/or 3′ direction of SEQ ID NOS:
- 3 to 5, and probes of 20 nucleotide bases or longer which contain at least 5 contiguous nucleotide bases from one of SEQ ID NOS:
- 1 or 2 continuous with from 1 to 15 flanking nucleotide bases in the 5′ and/or 3′ direction of SEQ ID NOS:
- 3 to 5.
3 more elements
Dependencies are inferred from the claim text.