Intact hepatitis c virus and the method for culturing it in a vitro cell culture
OwnerHengli Tang, Yonglie Chu +1inventors
InventorsHengli Tang, Yonglie Chu, Shulin Zhang
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Abstract
The present invention relates to the establishment of an in vitro cell culture system for culturing the whole HCV proliferating virus by using molecular biology and gene recombinant technology. Said method comprises the step of amplifying from the serum of HCV patients the full-length HCV genome comprising the 98 nucleotides at the 3′ end of the genome; site-specific mutating the NS5A and NS5B of HCV genome; inserting a marker gene IRES-GFP expression cassette into the NS5B 3′ end in the mutated HCV genome; and transfecting the sensitive cells and culturing, to obtain the infectious HCV offspring virus.
Claims3
A complete Hepatitis C Virus (HCV) strain, wherein the genotype of said virus is HCV 1b and said virus is deposited at CGMCC with deposition number of CGMCC. 0588.
Dependencies are inferred from the claim text.