Polypeptides containing polymorphisms of the repeated regions of pertactin in Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica, their use in diagnostics, and in immunogenic compositions

An immunogenic composition comprising a mixture of pertactins of Bordetella species or fragments thereof, wherein said pertactins or fragments thereof comprise at least two Bordetella bronchiseptica pertactin variants and wherein said pertactins of Bordetella species or fragments thereof are present in an amount sufficient to induce a humoral or immune response against Bordetella bronchiseptica in an animal to which the composition is administered.

An immunogenic composition comprising a mixture of pertactins of Bordetella species or fragments thereof, wherein said pertactins or fragments thereof comprise a mixture of Bordetella bronchiseptica pertactin variants, wherein each Bordetella bronchiseptica pertactin variant comprises 6, 7, 8, or 9 repeating PQP amino acid sequences in Region II thereof, and wherein said Bordetella bronchiseptica pertactin variants are present in amounts sufficient to induce a humoral or cellular immune response against Bordetella bronchiseptica in an animal to which the immunogenic composition is administered.

An immunogenic composition comprising a mixture of pertactins of Bordetella species or fragments thereof, wherein said pertactins or fragments thereof comprise a mixture of Bordetella bronchiseptica pertactin variants, wherein each Bordetella bronchiseptica pertactin variant comprises 1, 2, or 3 repeating GGXXP amino acid sequences in Region I thereof, and wherein said Bordetella bronchiseptica pertactin variants are present in amounts sufficient to induce a humoral or cellular immune response against Bordetella bronchiseptica in an animal to which the immunogenic composition is administered.

An immunogenic composition comprising a mixture of pertactins of Bordetella bronchiseptica species or fragments thereof, wherein said pertactins or fragments thereof comprise a mixture of Bordetella bronchiseptica pertactin variants, wherein at least one of said Bordetella bronchiseptica pertactin variants comprises Region II of pertactin of Bordetella bronchiseptica having 6, 7, 8, or 9 repeating PQP amino acid sequences in Region II thereof, and at least another of said Bordetella bronchiseptica pertactin variants comprises Region I of pertactin of Bordetella bronchiseptica having 1, 2, or 3 repeating GGXXP amino acid sequences in Region I thereof.

An immunogenic composition comprising a mixture of fragments of pertactins of Bordetella species, wherein said composition comprises:

• (a) pertactin of Bordetella parapertussis or a fragment thereof, and
• (b) pertactin of Bordetella bronchiseptica or a fragment thereof containing Region I, Region II, or both Region I and Region II,
• in amounts sufficient to induce a humoral or cellular immune response against Bordetella parapertussis and Bordetella bronchiseptica in an animal to which the immunogenic composition is administered.

A composition comprising a mixture of at least two Bordetella bronchiseptica pertactin variants, wherein each variant comprises Region II of a pertactin of Bordetella bronchiseptica, and wherein said variants differ from each other at least in the Region II they each comprise.

A composition comprising a mixture of Bordetella bronchiseptica pertactin variants, wherein each Bordetella bronchiseptica pertactin variant comprises 6, 7, 8, or 9 repeating PQP amino acid sequences in Region II thereof, and at least two Bordetella bronchiseptica pertactin variants differ in the number of said repeating PQP amino acid sequences contained therein.

A composition comprising a mixture of at least two Bordetella bronchiseptica pertactin variants, wherein each Bordetella bronchiseptica pertactin variant comprises Region I of pertactin of Bordetella bronchiseptica, and wherein said variants differ from each other at least in the Region I that each comprises.

A composition comprising a mixture of Bordetella bronchiseptica pertactin variants, wherein each Bordetella bronchiseptica pertactin variant comprises 1, 2, or 3 repeating GGXXP amino acid sequences in Region I thereof, and at least two Bordetella bronchiseptica pertactin variants differ in the number of said repeating GGXXP amino acid sequences contained therein.

A composition comprising a mixture of Bordetella bronchiseptica pertactin variants, wherein one of said Bordetella bronchiseptica pertactin variants comprises Region II of pertactin of Bordetella bronchiseptica and another of said Bordetella bronchiseptica pertactin variants comprises Region I of pertactin of Bordetella bronchiseptica.

A composition comprising a mixture of Bordetella bronchiseptica pertactin variants, wherein one of said Bordetella bronchiseptica pertactin variants comprises Region II of pertactin of Bordetella bronchiseptica having 6, 7, 8, or 9 repeating PQP amino acid sequences in Region II thereof, and another of said Bordetella bronchiseptica pertactin variants comprises Region I of pertactin of Bordetella bronchiseptica having 1, 2, or 3 repeating GGXXP amino acid sequences in Region I thereof.

A polypeptide comprising a sequence or a fragment of said sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, or SEQ ID NO:22.

A polypeptide consisting of the amino acids in SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, or SEQ ID NO:22.

An immunogenic composition consisting essentially of:

• (A) a polypeptide comprising Region I and Region II, or one polypeptide comprising Region I and one polypeptide comprising Region II, of a pertactin of Bordetella pertussis;
• (B) a polypeptide comprising Region I and Region II, or one polypeptide comprising Region I and one polypeptide comprising Region II, of a pertactin of Bordetella parapertussis;
• (C) a polypeptide comprising Region I and Region II, or one polypeptide comprising Region I and one polypeptide comprising Region II, of a pertactin of Bordetella bronchiseptica strain 9.73 and a polypeptide comprising Region I and Region II, or one polypeptide comprising Region I and one polypeptide comprising Region II, of a pertactin of Bordetella bronchiseptica of strain SEI.