Methods and reagent for preparing and using immunological agents specific for P-glycoprotein
OwnerEugene Mechetner, Igor B. Roninsoninventors
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Abstract
This invention relates to immunological reagents and methods specific for a mammalian, transmembrane protein termed Pgp, having a non-specific efflux pump activity established in the art as being a component of clinically-important multidrug resistance in cancer patients undergoing chemotherapy. The invention provides methods for developing and using immunological reagents specific for certain mutant forms of Pgp and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP depleting agents. The invention also provides improved methods for purifying hematopoietic stems cells expressing Pgp and diagnostic and therapeutic methods for cancer cells expressing Pgp.
Claims6
An immunological reagent produced by introducing a cell expressing a heterologous P-glycoprotein into an animal syngeneic with the species from which the cell was derived, wherein the heterologous P-glycoprotein is in a biochemical conformation adopted in the presence of Pgp-mediated transport substrates or ATP depleting agents, and producing immune cells in the animal expressing said reagent specific for P-glycoprotein in said biochemical conformation.
Dependencies are inferred from the claim text.