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OwnerNaoya Kobayashi, Noriaki Tanaka +4inventors
InventorsNaoya Kobayashi, Noriaki Tanaka, Teru Okitsu, Ji-Won Yoon, Hee-Sook Jun, Seungjin Shin
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The present invention provides a cell line which can be substituted for β cells in human mature pancreatic islets and express insulin in a glucose-concentration dependent manner, and enables the easy obtainment of the number of cells which meets the demand. The present invention also provides a therapeutical cell preparation for treating diabetes. The cell lines of the present invention can be obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human immortalized hepatic cell line FERM BP-7498 containing the TERT gene inserted in between a pair of LoxP sequences.
An immortalized hepatocyte cell line secreting insulin with glucose sensitivity, which is obtained by integrating both a nucleotide sequence encoding tamoxifen-induced Cre recombinase and a nucleotide sequence encoding insulin regulated by glucose-sensitive promoter into the chromosome in a human hepatic cell line FERM BP-7498 immortalized by the TERT gene inserted in between a pair of LoxP sequences.
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