Sensor for luminescense-optical determination of an analyte
AssigneeRoche Diagnostics Operations, Inc.
InventorIngo Klimant
Life of this patent
Estimating the term…
Abstract
The invention relates to an optochemical sensor functioning in accordance with the FRET-principle and exhibiting an acceptor (chromophore or luminophore) responsive to an analyte contained in a sample medium as well as a donor (luminophore), characterized in that acceptor and donor are located in separate chemical phases, whereby the phase containing the donor is essentially impermeable to the sample medium or to components of the sample medium affecting the luminescence characteristics of the luminophore. The invention further relates to a method for qualitative and/or quantitative determination of at least one analyte and/or component of a gaseous or liquid measuring medium according to the FRET-principle, characterized by the use of the sensor according to the invention.
Claims19
An optochemical sensor functioning in accordance with the FRET-principle and exhibiting an acceptor (chromophore or luminophore) responsive to an analyte contained in a sample medium as well as a donor (luminophore), characterized in that acceptor and donor are located in separate chemical phases, whereby the phase containing the donor is essentially impermeable to the sample medium or to components of the sample medium affecting the luminescence characteristics of the luminophore.
A sensor according to any of the preceding claims, characterized in that unplasticized polymers are used as a material for the donor phase.
A sensor according to any of the preceding claims, characterized in that polyvinyl chloride, polystyrenes, polycarbonates, polyethylenes, polyurethanes, silicones, copolymers of polyvinyl alcohol and polyvinyl acetate, copolymers of polyvinyl chloride, polyvinyl alcohol and polyvinyl acetate, cellulose, polyurethanes with hydrophilic groups, polyhydroxyethylmethacrylates, crosslinked polyvinyl alcohols, and/or polyacrylamides, optionally with up to 80% of plasticizer, are used as materials for the acceptor phase.
Dependencies are inferred from the claim text.