Method of separating compound(s) from mixture(s)
OwnerThierry Gandelheid, Anibal Lopesinventors
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Abstract
The invention relates to methods of separating compounds of interest from mixtures using high performance liquid chromatography (HPLC). An initial separation of a sample of a mixture is performed to predict a retention time and elution time for the compound of interest on a preparative scale HPLC column and, optionally, to verify that an expected compound of interest is present in the mixture. Preparative scale HPLC is then used to separate and collect at least a portion of the compound from the mixture using the predicted elution time of the compound.
Claims5
A method of separating a compound of interest from a mixture, the method comprising the steps of:
- (a) providing a mixture containing a compound of interest;
- (b) subjecting a portion of the mixture to a separation using thin layer chromatography to determine an Rf value for the compound of interest;
- (c) predicting an elution time of the compound of interest on a preparative scale HPLC column using the determined Rf value for the compound of interest;
- (d) subjecting all or a portion of the remaining mixture to a preparative scale HPLC system comprising a preparative scale HPLC column;
- and
- (e) collecting at least a portion of the compound of interest using the predicted elution time.
Dependencies are inferred from the claim text.