Chicken anaemia viruses of low pathogenicity
AssigneeAkzo Nobel N.V.
InventorsCarla Christina Schrier, Henricus Johannes Maria Jagt
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Abstract
The present invention provides a new antigenic type of chicken anaemia viruses (CAV). The CAV according to this invention is isolatable from turkeys in the field and its virulence for chickens is significantly reduced. This property makes these viruses particularly suited for preparing a live vaccine for protecting poultry against disease conditions resulting from CAV infection.
Claims13
1 A chicken anaemia virus (CAV), characterised in that the virus is neutralised by a reference sample comprising monoclonal antibody R2 secreted by a hybridoma cell line, a sample of which is deposited at the ECACC under accession no. 00020304.
2 A CAV according to claim 1, characterised in that the virus is in a live form.
3 A CAV according to claim 1 or 2, characterised in that the titre of the reference sample against the CAV is at least 10 (log2) per 50 μl, more preferably at least 12 (log2) per 50 μl or even at least 14 (log2) per 50 μl when examined against 300-1000 TCID50 per 50 μl of the CAV.
4 A CAV according to claim 3, characterised in that the titre of the reference sample against the CAV is at least 16 (log2) per 50 μl when examined against 300-1000 TCID50 per 50 μl of the CAV.
5 A CAV according to claim 4, characterised in that it is the CAV strain 319, a sample of which is deposited at the ECACC under accession no. 00012608.
6 A vaccine for protecting poultry against disease conditions resulting from a CAV infection, comprising a CAV according to claims 1-5, and a pharmaceutical acceptable carrier or diluent.
7 A vaccine according to claims 6, characterised in that the vaccine further comprises an adjuvant.
8 A vaccine according to claims 6 or 7, characterised in that the vaccine further comprises one or more vaccine components of other pathogens infectious to poultry.
9 A method for the preparation of CAV defined in claims 1-5 comprising the steps of:
- a. inoculating a susceptible substrate with the CAV,
- b. propagating the virus, and
- c. harvesting CAV containing material.
10 A method for the preparation of a vaccine for protecting poultry against disease conditions resulting from a CAV infection, comprising combining the harvested CAV obtained by the method according to claim 9, if desired after inactivation of the CAV, with a pharmaceutical acceptable carrier or diluent.
11 Use of a CAV defined in claims 1-5 for the manufacture of a vaccine for in ovo administration.
12 Use of a CAV defined in claims 1-5 for the manufacture of a vaccine for parenteral administration to one-day-old chicks.
13 A method for controlling disease conditions resulting from a CAV infection in poultry, comprising administering the vaccine according the claims 6-8 to the birds.